Evaluation of mtUPR and mito-nuclear imbalance in response to physical training: A multiorganic approach.

• Grant number: 16/01089-3
• Support Opportunities: Regular Research Grants
• Duration: September 01, 2016 - August 31, 2018
• Field of knowledge: Biological Sciences - Physiology - Physiology of Effort
• Principal Investigator: Eduardo Rochete Ropelle
• Grantee: Eduardo Rochete Ropelle
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated researchers: Dennys Esper Corrêa Cintra ; José Rodrigo Pauli

Abstract

The mitochondrial unfolded protein response (UPRmt), it is recognized as an adaptive intracellular pathway in response to stress, which ensures the integrity and function of the mitochondrial proteome through the mitochondrial chaperone and protease action. Mutagenic studies have shown that the disturbance caused by the stoichiometric imbalance between the mitochondrial DNA proteins (mtDNA) and proteins of the electron transport chain encoded by nuclear DNA (nDNA), is able to activate the UPRmt. In organisms including C. elegans and Drosophila the mitonuclear imbalance results in the improvement of cellular metabolism, mitochondrial function and increased longevity. The pharmacological induction of mitonuclear imbalance activates the UPRmt leading to increased biosynthesis and mitochondrial function, improving the oxidative capacity in tissues such as the liver and muscle, improving the functional capacity of these organs. Although the induction of UPRmt mediated mitonuclear imbalance mechanism appears to be a well-conserved biological process and which progresses in improvement in bodily functions, the effects of physical exercise on UPRmt and the mitonuclear imbalance have not yet been explored. Thus the current project are aimed at evaluating the UPRmt markers and mitonuclear imbalance in the hypothalamus, heart, liver and skeletal muscle of trained mice, relating this phenomenon to the mitochondrial function in these tissues. The development of this project will contribute significantly to the understanding of the role of UPRmt on organic adaptations induced by physical training. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRISOL, BARBARA M.; VEIGA, CAMILLA B.; LENHARE, LUCIENE; BRAGA, RENATA R.; SILVA, VAGNER R. R.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; MOURA, LEANDRO P.; PAULI, JOSE R.; ROPELLE, EDUARDO R.. Nicotinamide riboside induces a thermogenic response in lean mice. Life Sciences, v. 211, p. 1-7, OCT 15 2018. (16/05499-1, 16/01089-3)

SILVA, VAGNER R. R.; MICHELETTI, THAYANA O.; KATASHIMA, CARLOS K.; LENHARE, LUCIENE; MORARI, JOSEANE; MOURA-ASSIS, ALEXANDRE; DE LIMA-JUNIOR, JOSE C.; CAMARGO, JULIANA A.; PASSOS, GABRIELA R.; GASPAR, RODRIGO S.; et al. Exercise activates the hypothalamic S1PR1-STAT3 axis through the central action of interleukin 6 in mice. Journal of Cellular Physiology, v. 233, n. 12, p. 9426-9436, DEC 2018. (16/01089-3, 13/20052-5, 11/09656-0)

CRISOL, BARBARA M.; LENHARE, LUCIENE; GASPAR, RODRIGO S.; GASPAR, RAFAEL C.; MUNOZ, VITOR R.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; DE MOURA, LEANDRO P.; PAULI, JOSE R.; ROPELLE, EDUARDO R.. The role of physical exercise on Sestrin1 and 2 accumulations in the skeletal muscle of mice. Life Sciences, v. 194, p. 98-103, FEB 1 2018. (16/05499-1, 16/01089-3)

SILVA, VAGNER R. R.; KATASHIMA, CARLOS K.; LENHARE, LUCIENE; SILVA, CARLA G. B.; MORARI, JOSEANE; CAMARGO, RAFAEL L.; VELLOSO, LICIO A.; SAAD, MARIO A.; DA SILVA, ADELINO S. R.; PAULI, JOSE RODRIGO; et al. Chronic exercise reduces hypothalamic transforming growth factor-beta 1 in middle-aged obese mice. AGING-US, v. 9, n. 8, p. 1926-1940, AUG 2017. (16/01089-3)

LENHARE, LUCIENE; CRISOL, BARBARA M.; SILVA, VAGNER R. R.; KATASHIMA, CARLOS K.; CORDEIRO, ANDRE V.; PEREIRA, KARINA D.; LUCHESSI, AUGUSTO D.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; MOURA, LEANDRO P.; et al. Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice. Experimental Gerontology, v. 97, p. 17-21, OCT 15 2017. (16/01089-3)

CRISOL, BARBARA M.; VEIGA, CAMILLA B.; BRAGA, RENATA R.; LENHARE, LUCIENE; BAPTISTA, IGOR L.; GASPAR, RAFAEL C.; MUNOZ, VITOR R.; CORDEIRO, ANDRE V.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; et al. NAD(+)precursor increases aerobic performance in mice. EUROPEAN JOURNAL OF NUTRITION, v. 59, n. 6, p. 2427-2437, SEP 2020. (16/01089-3, 18/07634-9, 16/05499-1)