Cell culture models of fatty acid overload: Problems and solutions

Autor(es)

Alsabeeh, Nour ^{[1, 2, 3]} ; Chausse, Bruno ^[4] ; Kakimoto, Pamela A. ^[4] ; Kowaltowski, Alicia J. ^[4] ; Shirihai, Orian ^[1]

Afiliação do(s) autor(es)

^[1] UCLA, David Geffen Sch Med, Dept Med, Div Endocrinol Diabet & Hypertens, Los Angeles, CA 90095 - USA

^[2] Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 - USA

^[3] Kuwait Univ, Dept Physiol, Fac Med, Kuwait - Kuwait

^[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS; v. 1863, n. 2, p. 143-151, FEB 2018.
• Citações Web of Science: 10

Resumo

High plasma levels of fatty acids occur in a variety of metabolic diseases. Cellular effects of fatty acid overload resulting in negative cellular responses (lipotoxicity) are often studied in vitro, in an attempt to understand mechanisms involved in these diseases. Fatty acids are poorly soluble, and thus usually studied when complexed to albumins such as bovine serum albumin (BSA). The conjugation of fatty acids to albumin requires care pertaining to preparation of the solutions, effective free fatty acid concentrations, use of different fatty acid species, types of BSA, appropriate controls and ensuring cellular fatty acid uptake. This review discusses lipotoxicity models, the potential problems encountered when using these cellular models, as well as practical solutions for difficulties encountered. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 15/07670-7 - Perfis de ativação da micróglia e suas respectivas reprogramações metabólicas
• Beneficiário: Bruno Chausse de Freitas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/25862-0 - Bioenergética e sinalização redox hepática em modelo murino de obesidade: análise integrativa de estímulos nutricionais, hormonais e inflamatórios
• Beneficiário: Pâmela Aiako Hypólito Brito Kakimoto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto