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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cell culture models of fatty acid overload: Problems and solutions

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Author(s):
Alsabeeh, Nour [1, 2, 3] ; Chausse, Bruno [4] ; Kakimoto, Pamela A. [4] ; Kowaltowski, Alicia J. [4] ; Shirihai, Orian [1]
Total Authors: 5
Affiliation:
[1] UCLA, David Geffen Sch Med, Dept Med, Div Endocrinol Diabet & Hypertens, Los Angeles, CA 90095 - USA
[2] Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 - USA
[3] Kuwait Univ, Dept Physiol, Fac Med, Kuwait - Kuwait
[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Review article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS; v. 1863, n. 2, p. 143-151, FEB 2018.
Web of Science Citations: 10
Abstract

High plasma levels of fatty acids occur in a variety of metabolic diseases. Cellular effects of fatty acid overload resulting in negative cellular responses (lipotoxicity) are often studied in vitro, in an attempt to understand mechanisms involved in these diseases. Fatty acids are poorly soluble, and thus usually studied when complexed to albumins such as bovine serum albumin (BSA). The conjugation of fatty acids to albumin requires care pertaining to preparation of the solutions, effective free fatty acid concentrations, use of different fatty acid species, types of BSA, appropriate controls and ensuring cellular fatty acid uptake. This review discusses lipotoxicity models, the potential problems encountered when using these cellular models, as well as practical solutions for difficulties encountered. (AU)

FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/07670-7 - Study of the metabolic reprograming sustaining microglial activation to inflammatory profiles
Grantee:Bruno Chausse de Freitas
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/25862-0 - Hepatic bioenergetics and redox signaling in obesity murine model: integrative analysis of nutritional, hormonal, and inflammatory stimuli
Grantee:Pâmela Aiako Hypólito Brito Kakimoto
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)