Exercise Increases Insulin Sensitivity and Skeletal Muscle AMPK Expression in Systemic Lupus Erythematosus: A Randomized Controlled Trial

Autor(es):Mostrar menos -

Benatti, Fabiana B. ^{[1, 2]} ; Miyake, Cintia N. H. ^[1] ; Dantas, Wagner S. ^[1] ; Zambelli, Vanessa O. ^[3] ; Shinjo, Samuel K. ^[1] ; Pereira, Rosa M. R. ^[1] ; Silva, Maria Elizabeth R. ^[4] ; Sa-Pinto, Ana Lucia ^[1] ; Borba, Eduardo ^[1] ; Bonfa, Eloisa ^[1] ; Gualano, Bruno ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med FMUSP, Hosp Clin HCFMUSP, Div Rheumatol, Sao Paulo - Brazil

^[2] Univ Estadual Campinas UNICAMP, Sch Appl Sci, Sao Paulo - Brazil

^[3] Butantan Inst, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med FMUSP, Hosp Clin HCFMUSP, Div Endocrinol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN IMMUNOLOGY; v. 9, APR 27 2018.
• Citações Web of Science: 3

Resumo

Systemic lupus erythematosus (SLE) patients may show increased insulin resistance (IR) when compared with their healthy peers. Exercise training has been shown to improve insulin sensitivity in other insulin-resistant populations, but it has never been tested in SLE. Therefore, the aim of the present study was to assess the efficacy of a moderate-intensity exercise training program on insulin sensitivity and potential underlying mechanisms in SLE patients with mild/inactive disease. A 12-week, randomized controlled trial was conducted. Nineteen SLE patients were randomly assigned into two groups: trained (SLE-TR, n = 9) and non-trained (SLE-NT, n = 10). Before and after 12 weeks of the exercise training program, patients underwent a meal test (MT), from which surrogates of insulin sensitivity and beta-cell function were determined. Muscle biopsies were performed after the MT for the assessment of total and membrane GLUT4 and proteins related to insulin signaling {[} Akt and AMP-activated protein kinase (AMPK)]. SLE-TR showed, when compared with SLE-NT, significant decreases in fasting insulin {[}-39 vs. + 14%, p = 0.009, effect size (ES) = -1.0] and in the insulin response to MT (-23 vs. + 21%, p = 0.007, ES = -1.1), homeostasis model assessment IR (-30 vs. + 15%, p = 0.005, ES = -1.1), a tendency toward decreased proinsulin response to MT (-19 vs. + 6%, p = 0.07, ES = -0.9) and increased glucagon response to MT (+3 vs. -3%, p = 0.09, ES = 0.6), and significant increases in the Matsuda index (+66 vs. -31%, p = 0.004, ES = 0.9) and fasting glucagon (+4 vs. -8%, p = 0.03, ES = 0.7). No significant differences between SLT-TR and SLT-NT were observed in fasting glucose, glucose response to MT, and insulinogenic index (all p > 0.05). SLE-TR showed a significant increase in AMPK Thr 172 phosphorylation when compared to SLE-NT (+73 vs. -12%, p = 0.014, ES = 1.3), whereas no significant differences between groups were observed in Akt Ser 473 phosphorylation, total and membrane GLUT4 expression, and GLUT4 translocation (all p > 0.05). In conclusion, a 12-week moderate-intensity aerobic exercise training program improved insulin sensitivity in SLE patients with mild/inactive disease. This effect appears to be partially mediated by the increased insulin-stimulated skeletal muscle AMPK phosphorylation. (AU)

• Processo FAPESP: 11/08302-0 - Efeito do exercício físico aeróbio crônico na sensibilidade à insulina de pacientes com lúpus eritematoso sistêmico
• Beneficiário: Fabiana Braga Benatti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/03756-4 - Avaliação da relevância dos níveis sanguíneos de drogas utilizadas em doenças autoimunes reumatológicas no acompanhamento da segurança, eficácia e aderência à terapêutica
• Beneficiário: Eloisa Silva Dutra de Oliveira Bonfá
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/24093-2 - Efeito do exercício físico aeróbio no perfil inflamatório e na sensibilidade à insulina de pacientes com lúpus eritematoso sistêmico
• Beneficiário: Eloisa Silva Dutra de Oliveira Bonfá
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/02546-1 - Efeitos metabólicos, fisiológicos e moleculares da interrupção do tempo sedentário em pacientes com artrite reumatoide: um estudo crossover e randomizado
• Beneficiário: Fabiana Braga Benatti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado