Amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze

Autor(es)

Sorregotti, Tatiani ^{[1, 2]} ; Cipriano, Ana Claudia ^{[1, 2]} ; Cruz, Fabio Cardoso ^[3] ; Mascarenhas, Diego Cardozo ^{[1, 2]} ; Rodgers, Robert John ^[4] ; Nunes-de-Souza, Ricardo Luiz ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] UNESP, UFSCar, Joint Grad Program Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Lab Neuropsychopharmacol, BR-14800903 Araraquara, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Pharmacol, BR-04023901 Sao Paulo, SP - Brazil

^[4] Univ Leeds, Sch Psychol, Leeds LS2 9JT, W Yorkshire - England

• Tipo de documento: Artigo Científico
• Fonte: Behavioural Brain Research; v. 338, p. 159-165, FEB 15 2018.
• Citações Web of Science: 5

Resumo

Previous studies have shown that the exposure to an open elevated plus maze (oEPM, an EPM with all four open arms) elicits fear/anxiety-related responses in laboratory rodents. However, very little is known about the underlying neural substrates of these defensive behaviors. Accordingly, the present study investigated the effects of chemical inactivation of the amygdala {[}through local injection of cobalt chloride (CoC12: a nonspecific synaptic blocker)] on the behavior of oEPM-exposed mice. In a second experiment, the pattern of activation of the basolateral (BLA) and central (CeA) nuclei of the amygdala was assessed through quantification of Fos protein expression in mice subjected to one of several behavioral manipulations. To avoid the confound of acute handling stress, 4 independent groups of mice were habituated daily for 10 days to an enclosed EPM (eEPM) and, on day 11 prior to immunohistochemistry, were either taken directly from their home cage (control) or individually exposed for 10 min to a new clean holding cage (novelty); an eEPM, or the oEPM. An additional group of mice (maze-na ve) was not subjected to either the habituation or exposure phase but were simply chosen at random from their home cages to undergo an identical immunohistochemistry procedure. Results showed that amygdala inactivation produced an anxiolytic-like profile comprising reductions in time spent in the proximal portions of the open arms and total stretched attend postures (SAP) as well as increases in time spent in the distal portions of the open arms and total head-dipping. Moreover, Fos-positive labeled cells were bilaterally increased in the amygdaloid complex, particularly in the BLA, of oEPM-exposed animals compared to all other groups. These results suggest that the amygdala (in particular, its BLA nucleus) plays a key role in the modulation of defensive behaviors in oEPM-exposed mice. (AU)

• Processo FAPESP: 13/06764-2 - A antinocicepção induzida pela derrota social: implicações da neurotransmissão vaniloide espinal e supraespinal em camundongos.
• Beneficiário: Diego Cardozo Mascarenhas
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/24986-2 - Participação de pequenos grupos neurais seletivamente ativados (neuronal ensembles) na reinstalação da autoadministração de etanol induzida pelo ambiente: investigação farmacogenética, optogenética e molecular
• Beneficiário: Fabio Cardoso Cruz
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/01283-6 - Sistema hierárquico de defesa no camundongo: modulação pelo fator de liberação de corticotrofina (CRF)
• Beneficiário: Ricardo Luiz Nunes de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/04561-1 - Avaliação neuropsicofarmacológica dos mecanismos Crf-érgicos na amídala, nas reações de defesa de camundongos pré-expostos à derrota social
• Beneficiário: Ana Cláudia Cipriano
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 14/02956-7 - Papel da amídala na antinocicepção induzida pelo medo: avaliações comportamental, imunoistoquímica e farmacológica
• Beneficiário: Tatiani Sorregotti
• Modalidade de apoio: Bolsas no Brasil - Doutorado