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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Amygdaloid involvement in the defensive behavior of mice exposed to the open elevated plus-maze

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Author(s):
Sorregotti, Tatiani [1, 2] ; Cipriano, Ana Claudia [1, 2] ; Cruz, Fabio Cardoso [3] ; Mascarenhas, Diego Cardozo [1, 2] ; Rodgers, Robert John [4] ; Nunes-de-Souza, Ricardo Luiz [1, 2]
Total Authors: 6
Affiliation:
[1] UNESP, UFSCar, Joint Grad Program Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Sch Pharmaceut Sci, Lab Neuropsychopharmacol, BR-14800903 Araraquara, SP - Brazil
[3] Univ Fed Sao Paulo, Dept Pharmacol, BR-04023901 Sao Paulo, SP - Brazil
[4] Univ Leeds, Sch Psychol, Leeds LS2 9JT, W Yorkshire - England
Total Affiliations: 4
Document type: Journal article
Source: Behavioural Brain Research; v. 338, p. 159-165, FEB 15 2018.
Web of Science Citations: 5
Abstract

Previous studies have shown that the exposure to an open elevated plus maze (oEPM, an EPM with all four open arms) elicits fear/anxiety-related responses in laboratory rodents. However, very little is known about the underlying neural substrates of these defensive behaviors. Accordingly, the present study investigated the effects of chemical inactivation of the amygdala {[}through local injection of cobalt chloride (CoC12: a nonspecific synaptic blocker)] on the behavior of oEPM-exposed mice. In a second experiment, the pattern of activation of the basolateral (BLA) and central (CeA) nuclei of the amygdala was assessed through quantification of Fos protein expression in mice subjected to one of several behavioral manipulations. To avoid the confound of acute handling stress, 4 independent groups of mice were habituated daily for 10 days to an enclosed EPM (eEPM) and, on day 11 prior to immunohistochemistry, were either taken directly from their home cage (control) or individually exposed for 10 min to a new clean holding cage (novelty); an eEPM, or the oEPM. An additional group of mice (maze-na ve) was not subjected to either the habituation or exposure phase but were simply chosen at random from their home cages to undergo an identical immunohistochemistry procedure. Results showed that amygdala inactivation produced an anxiolytic-like profile comprising reductions in time spent in the proximal portions of the open arms and total stretched attend postures (SAP) as well as increases in time spent in the distal portions of the open arms and total head-dipping. Moreover, Fos-positive labeled cells were bilaterally increased in the amygdaloid complex, particularly in the BLA, of oEPM-exposed animals compared to all other groups. These results suggest that the amygdala (in particular, its BLA nucleus) plays a key role in the modulation of defensive behaviors in oEPM-exposed mice. (AU)

FAPESP's process: 13/06764-2 - Social defeat-induced antinociception: implication of spinal and supraspinal vanilloid neurotransmission in mice.
Grantee:Diego Cardozo Mascarenhas
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/02956-7 - Role of the amygdala in fear-induced antinociception: behavioral, pharmacological and immunohistochemical assessments
Grantee:Tatiani Sorregotti
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/04561-1 - Neuropsychopharmacological evaluation of the CRF mechanisms located within the amygdala on defensive reactions induced by prior exposure to social defeat in mice
Grantee:Ana Cláudia Cipriano
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 13/01283-6 - Hierarchic defensive system in mice: role of the corticotrophin-releasing factor (CRF)
Grantee:Ricardo Luiz Nunes de Souza
Support type: Regular Research Grants
FAPESP's process: 13/24986-2 - The role of neuronal ensembles in context-induced reinstatement of ethanol seeking: pharmacogenetic, optogenetic and molecular investigation
Grantee:Fabio Cardoso Cruz
Support type: Research Grants - Young Investigators Grants