Polyamine- and Amino Acid-Related Metabolism: The Roles of Arginine and Ornithine are Associated with the Embryogenic Potential

Autor(es)

de Oliveira, Leandro Francisco ^[1] ; Navarro, Bruno Viana ^[1] ; Cerruti, Giovanni Victorio ^[1] ; Elbl, Paula ^[1] ; Minocha, Rakesh ^[2] ; Minocha, Subhash C. ^[3] ; Wendt dos Santos, Andre Luis ^[1] ; Segal Floh, Eny Iochevet ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biosci, Dept Bot, Lab Plant Cell Biol, Rua Matao 277, Room 107, BR-05508090 Sao Paulo, SP - Brazil

^[2] US Forest Serv, USDA, Northern Res Stn, 271 Mast Rd, Durham, NH 03824 - USA

^[3] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 - USA

• Tipo de documento: Artigo Científico
• Fonte: Plant and Cell Physiology; v. 59, n. 5, p. 1084-1098, MAY 2018.
• Citações Web of Science: 4

Resumo

The mechanisms that control polyamine (PA) metabolism in plant cell lines with different embryogenic potential are not well understood. This study involved the use of two Araucaria angustifolia cell lines, one of which was defined as being blocked, in that the cells were incapable of developing somatic embryos, and the other as being responsive, as the cells could generate somatic embryos. Cellular PA metabolism was modulated by using 5 mM arginine (Arg) or ornithine (Orn) at two time points during cell growth. Two days after subculturing with Arg, an increase in citrulline (Cit) content was observed, followed by a higher expression of genes related to PA catabolism in the responsive cell line; whereas, in the blocked cell line, we only observed an accumulation of PAs. After 14 d, metabolism was directed towards putrescine accumulation in both cell lines. Exogenous Arg and Orn not only caused a change in cellular contents of PAs, but also altered the abundance of a broader spectrum of amino acids. Specifically, Cit was the predominant amino acid. We also noted changes in the expression of genes related to PA biosynthesis and catabolism. These results indicate that Arg and Orn act as regulators of both biosynthetic and catabolic PA metabolites; however, we suggest that they have distinct roles associated with embryogenic potential of the cells. (AU)

• Processo FAPESP: 14/26888-0 - Marcadores bioquímicos associados ao metabolismo de carboidratos durante a embriogênese zigótica e somática de Araucaria angustifolia (Bert.) O. Ktze.
• Beneficiário: Bruno Viana Navarro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 15/21075-4 - Análises fisiológicas e do S-nitroproteoma durante a embriogênese in vivo e in vitro do Pinheiro brasileiro (Araucaria angustifolia (Bertol.) Kuntze)
• Beneficiário: André Luiz Wendt dos Santos
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/22738-9 - Metabolismo de poliaminas na embriogênese zigótica e somática de Araucaria angustifolia (Bert.) O. Kuntze
• Beneficiário: Leandro Francisco de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado