Comparative proteomic analysis of Xanthomonas citri ssp citri periplasmic proteins reveals changes in cellular envelope metabolism during in vitro pathogenicity induction

Autor(es):Mostrar menos -

Artier, Juliana ^[1] ; Zandonadi, Flavia da Silva ^[1] ; de Souza Carvalho, Flavia Maria ^[2] ; Pauletti, Bianca Alves ^[3] ; Paes Leme, Adriana Franco ^[3] ; Carnielli, Carolina Moretto ^[1] ; Selistre-de-Araujo, Heloisa Sobreiro ^[4] ; Bertolini, Maria Celia ^[5] ; Ferro, Jesus Aparecido ^[2] ; Belasque Junior, Jose ^[6] ; Franco de Oliveira, Julio Cezar ^[7] ; Marques Novo-Mansur, Maria Teresa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, UFSCar, Dept Genet & Evolucao, Lab Bioquim & Biol Mol Aplicada, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet Jaboticabal, Dept Tecnol, BR-14884900 Jaboticabal, SP - Brazil

^[3] CNPEM, LNBio, Lab Espectrometria Massas, Lab Nacl Biociencias, BR-13083970 Campinas, SP - Brazil

^[4] Univ Fed Sao Carlos, UFSCar, Dept Ciencias Fisiol, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Estadual Paulista, UNESP, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800060 Araraquara, SP - Brazil

^[6] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

^[7] Univ Fed Sao Paulo, UNIFESP, Dept Ciencias Biol, Lab Interacoes Microbianas, BR-09913030 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR PLANT PATHOLOGY; v. 19, n. 1, p. 143-157, JAN 2018.
• Citações Web of Science: 3

Resumo

Citrus canker is a plant disease caused by Gram-negative bacteria from the genus Xanthomonas. The most virulent species is Xanthomonas citri ssp. citri (XAC), which attacks a wide range of citrus hosts. Differential proteomic analysis of the periplasm-enriched fraction was performed for XAC cells grown in pathogenicity-inducing (XAM-M) and pathogenicity-non-inducing (nutrient broth) media using two-dimensional electrophoresis combined with liquid chromatography-tandem mass spectrometry. Amongst the 40 proteins identified, transglycosylase was detected in a highly abundant spot in XAC cells grown under inducing condition. Additional up-regulated proteins related to cellular envelope metabolism included glucose-1-phosphate thymidylyltransferase, dTDP-4-dehydrorhamnose-3,5-epimerase and peptidyl-prolyl cis-trans-isomerase. Phosphoglucomutase and superoxide dismutase proteins, known to be involved in pathogenicity in other Xanthomonas species or organisms, were also detected. Western blot and quantitative real-time polymerase chain reaction analyses for transglycosylase and superoxide dismutase confirmed that these proteins were up-regulated under inducing condition, consistent with the proteomic results. Multiple spots for the 60-kDa chaperonin and glyceraldehyde-3-phosphate dehydrogenase were identified, suggesting the presence of post-translational modifications. We propose that substantial alterations in cellular envelope metabolism occur during the XAC infectious process, which are related to several aspects, from defence against reactive oxygen species to exopolysaccharide synthesis. Our results provide new candidates for virulence-related proteins, whose abundance correlates with the induction of pathogenicity and virulence genes, such as hrpD6, hrpG, hrpB7, hpa1 and hrpX. The results present new potential targets against XAC to be investigated in further functional studies. (AU)

• Processo FAPESP: 07/50910-2 - Análise proteômica diferencial em Xanthomonas axonopodis: proteínas e genes de interesse biotecnológico
• Beneficiário: Maria Teresa Marques Novo Mansur
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores