Conditional Deletion of Ric-8b in Olfactory Sensory Neurons Leads to Olfactory Impairment

Autor(es)

Machado, Cleiton F. ^[1] ; Nagai, Maria H. ^[1] ; Lyra, Cassandra S. ^[1] ; Reis-Silva, Thiago M. ^[2] ; Machado, Andre M. ^[3] ; Glezer, Isaias ^[3] ; Felicio, Luciano F. ^[2] ; Malnic, Bettina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Vet Med, Dept Pathol, BR-05508270 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NEUROSCIENCE; v. 37, n. 50, p. 12202-12213, DEC 13 2017.
• Citações Web of Science: 3

Resumo

The olfactory system can discriminate a vast number of odorants. This ability derives from the existence of a large family of odorant receptors expressed in the cilia of the olfactory sensory neurons. Odorant receptors signal through the olfactory-specific G-protein subunit, G alpha olf. Ric-8b, a guanine nucleotide exchange factor, interacts with G alpha olf and can amplify odorant receptor signal transduction in vitro. To explore the function of Ric-8b in vivo, we generated a tissue specific knock-out mouse by crossing OMP-Cre transgenic mice to Ric-8b floxed mice. We found that olfactory-specific Ric-8b knock-out mice of mixed sex do not express the G alpha olf protein in the olfactory epithelium. We also found that in these mice, the mature olfactory sensory neuron layer is reduced, and that olfactory sensory neurons show increased rate of cell death compared with wild-type mice. Finally, behavioral tests showed that the olfactory-specific Ric-8b knock-out mice show an impaired sense of smell, even though their motivation and mobility behaviors remain normal. (AU)

• Processo FAPESP: 16/24471-0 - Receptores olfatórios: mecanismos de expressão gênica e transdução de sinal
• Beneficiário: Bettina Malnic
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/15495-8 - Identificação dos processos celulares que estão alterados no knockout de Ric-8B utilizando sequenciamento em larga escala de transcriptomas
• Beneficiário: Maíra Harume Nagai
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/24640-6 - Papel da proteína Ric-8B em neurônios olfatórios
• Beneficiário: Cleiton Fagundes Machado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado