Molecular basis of neuronal diversity in olfaction and its functional implications
Odorant receptors: mechanisms of gene expression and signal transduction
Identification of the cellular processes altered in Ric-8B knockout mice by using ...
| Full text | |
| Author(s): |
Machado, Cleiton F.
[1]
;
Nagai, Maria H.
[1]
;
Lyra, Cassandra S.
[1]
;
Reis-Silva, Thiago M.
[2]
;
Machado, Andre M.
[3]
;
Glezer, Isaias
[3]
;
Felicio, Luciano F.
[2]
;
Malnic, Bettina
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Vet Med, Dept Pathol, BR-05508270 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biochem, BR-04023900 Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | JOURNAL OF NEUROSCIENCE; v. 37, n. 50, p. 12202-12213, DEC 13 2017. |
| Web of Science Citations: | 3 |
| Abstract | |
The olfactory system can discriminate a vast number of odorants. This ability derives from the existence of a large family of odorant receptors expressed in the cilia of the olfactory sensory neurons. Odorant receptors signal through the olfactory-specific G-protein subunit, G alpha olf. Ric-8b, a guanine nucleotide exchange factor, interacts with G alpha olf and can amplify odorant receptor signal transduction in vitro. To explore the function of Ric-8b in vivo, we generated a tissue specific knock-out mouse by crossing OMP-Cre transgenic mice to Ric-8b floxed mice. We found that olfactory-specific Ric-8b knock-out mice of mixed sex do not express the G alpha olf protein in the olfactory epithelium. We also found that in these mice, the mature olfactory sensory neuron layer is reduced, and that olfactory sensory neurons show increased rate of cell death compared with wild-type mice. Finally, behavioral tests showed that the olfactory-specific Ric-8b knock-out mice show an impaired sense of smell, even though their motivation and mobility behaviors remain normal. (AU) | |
| FAPESP's process: | 16/24471-0 - Odorant receptors: mechanisms of gene expression and signal transduction |
| Grantee: | Bettina Malnic |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/15495-8 - Identification of the cellular processes altered in Ric-8B knockout mice by using transcriptome deep sequencing |
| Grantee: | Maíra Harume Nagai |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/24640-6 - Ric-8B protein in olfactory sensory neurons |
| Grantee: | Cleiton Fagundes Machado |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |