Hypocholesterolaemic effect of rat-administered oral doses of the isolated 7S globulins from cowpeas and adzuki beans

Autor(es)

Ferreira, Ederlan S. ^[1] ; Amaral, Ana Lucia S. ^[2] ; Demonte, Aureluce ^[2] ; Zanelli, Cleslei F. ^[3] ; Capraro, Jessica ^[4] ; Duranti, Marcello ^[4] ; Neves, Valdir A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Bahia, Sch Pharm, Dept Bromatol Anal, BR-40170115 Salvador, BA - Brazil

^[2] Sao Paulo State Univ, Dept Food & Nutr, BR-14801902 Araraquara, SP - Brazil

^[3] Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP - Brazil

^[4] Univ Milan, Dept Food Environm & Nutr Sci DeFENS, Via G Celoria 2, I-20133 Milan - Italy

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL SCIENCE; v. 4, FEB 16 2015.
• Citações Web of Science: 11

Resumo

The role of seed proteins, especially soyabean 7S globulins, in controlling dyslipidaemia is widely acknowledged. Amino acid sequence homology among the proteins of this family could reflect similar biological functions in other species. The aim of the present study was to unveil a hypolipidaemic effect of the 7S globulins from cowpeas (7S-C) and adzuki beans (7S-A), administered orally to rats fed a hypercholesterolaemic (HC; high cholesterol and TAG) diet for 28 d. A total of forty-five rats were divided into five groups (nine rats per group): (1) standard (STD) diet; (2) HC diet; (3) HC diet + 7S-C (300 mg/kg per d); (4) HC diet + 7S-A (300 mg/kg per d); and (5) HC diet + simvastatin (SVT; 50 mg/kg per d), as a control. Significant decreases in food intake and final body weight of rats receiving HC + 7S-C and HC + 7S-A diets compared with groups fed the HC and STD diets were observed. Significant decreases in serum total and non-HDL-cholesterol of 7S-C, 7S-A and SVT groups were also observed. HDL-cholesterol levels increased in the 7S-C, 7S-A and SVT groups, while hepatic cholesterol and TAG concentrations were significantly lower than in the HC diet group for the 7S-C-supplemented group only. Faecal excretions of fat and cholesterol in HC diet groups were considerably higher in animals consuming the 7S globulins. The results show that cowpea and adzuki bean 7S globulins promote cholesterol-decreasing effects in hypercholesterolaemic rats even at low dosages, as already observed for other legume seed storage proteins of this family. This main effect is discussed in relation to the possible mechanisms of action. (AU)

• Processo FAPESP: 11/11779-3 - Efeito de vicilinas isoladas das espécies Glycine max, Vigna unguiculata e Vigna angularis no metabolismo do colesterol em ratos
• Beneficiário: Valdir Augusto Neves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/11511-0 - Efeito de vicilinas isoladas das espécies Glycine max, Vigna unguiculata e V. angularis no metabolismo lipídico de ratos
• Beneficiário: Ederlan de Souza Ferreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado