Evaluation of aluminum phthalocyanine chloride and DNA interactions for the design of an advanced drug delivery system in photodynamic therapy

Autor(es)

Jayme, Cristiano Ceron ^[1] ; Calori, Italo Rodrigo ^[2] ; Freire Cunha, Elise Marques ^[3] ; Tedesco, Antonio Claudio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Photo Biol & Photomed Res Grp, Dept Chem, Ctr Nanotechnol & Tissue Engn, Av Bandeirantes 3900, BR-14040901 Sao Paulo - Brazil

^[2] State Univ Maringo, Res Nucleus Photodynam Therapy, Dept Chem, Av Colombo 5790, BR-97020900 Maringa, Parana - Brazil

^[3] Fed Inst Rondonia, Campus Ji Parand, Rua Rio Amazonas 151, BR-76900730 Ji Parana, Rondonia - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 201, p. 242-248, AUG 5 2018.
• Citações Web of Science: 4

Resumo

The aim of this study was to evaluate the interaction of aluminum phthalocyanine chloride (AlClPc) with double stranded DNA. Absorption and fluorescence spectra, resonance light scattering, and circular dichroism were evaluated in water and water/ethanol mixtures with different concentrations of DNA or AlClPc. AlClPc showed a high ability to bind to DNA in both water and 4/6 water/ethanol mixture (v/v), with a majority of monomeric and aggregated initial forms of AlClPc, respectively. In this interaction, AlClPc bound preferentially to the grooves of DNA. The monomeric/aggregate state of AlClPc in DNA was dependent on the AlClPc/DNA ratio. At low concentrations of AlClPc, the interaction of AlClPc with few DNA sites caused a curvature in the DNA structure that provided a favorable environment for the intercalation of AlClPc aggregates. Increase in AlClPc concentration induced interactions with a high number of binding sites on DNA, which prevented bending and therefore aggregation of AlClPc molecules throughout the double-stranded DNA. These results are relevant to the understanding of the behavior and interaction of AlClPc with double-stranded DNA in the design of novel drug delivery systems for clinical application in photodynamic therapy as a new approach to treat skin or oral cancer, scars, or wound healing. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Linha de fomento: Auxílio à Pesquisa - Temático