Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers

Autor(es)

Granato, Alessandro E. C. ^[1] ; Ribeiro, Andre C. ^[2] ; Marciano, Fernanda R. ^{[2, 3]} ; Rodrigues, Bruno V. M. ^{[2, 4]} ; Lobo, Anderson O. ^{[5, 2, 6]} ; Porcionatto, Marimelia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Neurobiol Lab, Dept Biochem, Sao Paulo - Brazil

^[2] Univ Brasil, Inst Sci & Technol, Sao Paulo, SP - Brazil

^[3] Northeastern Univ, Dept Civil Engn, 420 Snell Engn Ctr, Boston, MA 02115 - USA

^[4] Inst Tecnol Aeronaut, Plasma & Proc Lab, Sao Jose Dos Campos, SP - Brazil

^[5] MIT, Dept Chem, Cambridge, MA 02139 - USA

^[6] Univ Fed Piaui, Technol Ctr, Mat Sci & Engn Grad Program, Interdisciplinary Lab Adv Mat, Teresina, PI - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Nanomedicine-Nanotechnology Biology and Medicine; v. 14, n. 6, p. 1753-1763, AUG 2018.
• Citações Web of Science: 1

Resumo

We present a methodology for production and application of electrospun hybrid materials containing commercial polyester (poly (butylene adipate-co-terephthalate; PBAT), and a conductive polymer (polypyrrole; PPy) as scaffold for neuronal growth and differentiation. The physical-chemical properties of the scaffolds and optimization of the electrospinning parameters are presented. The electrospun scaffolds are biocompatible and allow proper adhesion and spread of mesenchymal stem cells (MSCs). Fibers produced with PBAT with or without PPy were used as scaffold for Neuro2a mouse neuroblastoma cells adhesion and differentiation. Neuro2a adhered to PBAT and PBAT/PPy2% scaffolds without laminin coating. However, Neuro2a failed to differentiate in PBAT when stimulated by treatment with retinoic acid (RA), but differentiated in PBAT/PPy2% fibers. We hypothesize that PBAT hydrophobicity inhibited proper spreading and further differentiation, and inhibition was overcome by coating the PBAT fibers with laminin. We conclude that fibers produced with the combination of PBAT and PPy can support neuronal differentiation. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/00652-5 - Estudo dos mecanismos moleculares da migração, sobrevivência e diferenciação de células-tronco neurais
• Beneficiário: Marimélia Aparecida Porcionatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores