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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polypyrrole increases branching and neurite extension by Neuro2A cells on PBAT ultrathin fibers

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Author(s):
Granato, Alessandro E. C. [1] ; Ribeiro, Andre C. [2] ; Marciano, Fernanda R. [2, 3] ; Rodrigues, Bruno V. M. [2, 4] ; Lobo, Anderson O. [5, 2, 6] ; Porcionatto, Marimelia [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Escola Paulista Med, Neurobiol Lab, Dept Biochem, Sao Paulo - Brazil
[2] Univ Brasil, Inst Sci & Technol, Sao Paulo, SP - Brazil
[3] Northeastern Univ, Dept Civil Engn, 420 Snell Engn Ctr, Boston, MA 02115 - USA
[4] Inst Tecnol Aeronaut, Plasma & Proc Lab, Sao Jose Dos Campos, SP - Brazil
[5] MIT, Dept Chem, Cambridge, MA 02139 - USA
[6] Univ Fed Piaui, Technol Ctr, Mat Sci & Engn Grad Program, Interdisciplinary Lab Adv Mat, Teresina, PI - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Nanomedicine-Nanotechnology Biology and Medicine; v. 14, n. 6, p. 1753-1763, AUG 2018.
Web of Science Citations: 1
Abstract

We present a methodology for production and application of electrospun hybrid materials containing commercial polyester (poly (butylene adipate-co-terephthalate; PBAT), and a conductive polymer (polypyrrole; PPy) as scaffold for neuronal growth and differentiation. The physical-chemical properties of the scaffolds and optimization of the electrospinning parameters are presented. The electrospun scaffolds are biocompatible and allow proper adhesion and spread of mesenchymal stem cells (MSCs). Fibers produced with PBAT with or without PPy were used as scaffold for Neuro2a mouse neuroblastoma cells adhesion and differentiation. Neuro2a adhered to PBAT and PBAT/PPy2% scaffolds without laminin coating. However, Neuro2a failed to differentiate in PBAT when stimulated by treatment with retinoic acid (RA), but differentiated in PBAT/PPy2% fibers. We hypothesize that PBAT hydrophobicity inhibited proper spreading and further differentiation, and inhibition was overcome by coating the PBAT fibers with laminin. We conclude that fibers produced with the combination of PBAT and PPy can support neuronal differentiation. (C) 2018 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 11/20345-7 - Study of nanoparticle-incorporated diamond-like carbon films for biomedical applications
Grantee:Fernanda Roberta Marciano
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 12/00652-5 - Molecular mechanisms of neural stem cells migration, survival and differentiation
Grantee:Marimélia Aparecida Porcionatto
Support Opportunities: Regular Research Grants
FAPESP's process: 11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration
Grantee:Anderson de Oliveira Lobo
Support Opportunities: Research Grants - Young Investigators Grants