Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment

Autor(es)

Petrillo, R. ^[1] ; Eloy, J. O. ^[2] ; Paschoal, J. A. R. ^[1] ; Lopez, V, R. F.

Afiliação do(s) autor(es)

^[1] V, Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Pharmazie; v. 73, n. 3, p. 133-138, MAR 2018.
• Citações Web of Science: 1

Resumo

5-Fluorouracil (5-FU) is used for the topical treatment of pre-cancerous skin lesions. However, previous reports focus on 5-FU quantification in plasma samples, failing to provide information about drug quantification in the skin. Therefore, the aim of this work was to develop a simple and reliable analytical method employing HPLC-UV for 5-FU quantification in skin samples. Chromatographic separation was obtained using the commonly available Lichrospher RP-C18 endcapped column. Porcine ear skin matrix-based analytical curves with thymine, as internal standard, were used. 5-FU was eluted at 5.2 min and thymine at 13.0 min. The method was selective, precise and accurate in the linear range from 0.3 to 6 mu g/mL. The samples were stable after three cycles of freeze/thawing and extraction efficiency rates above 95% were obtained. In vitro skin penetration studies of an aqueous solution and a commercial cream of 5-FU were performed. The cream improved 5-FU retention into the skin and permeation through the skin compared to the solution. Therefore, the method developed herein can be applied to the study of formulations for topical delivery of 5-FU. (AU)

• Processo FAPESP: 14/22451-7 - Sistemas de liberação sustentada e direcionada de fármacos para o tecido epitelial
• Beneficiário: Renata Fonseca Vianna Lopez
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/15134-2 - Preparo de imunolipossomas para o tratamento tópico de câncer de pele
• Beneficiário: Raquel Petrilli
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 12/23764-3 - Aplicação tópica de lipossomas contendo cetuximabe: efeito do uso de métodos físicos de penetração cutânea no carcinoma celular escamoso de pele
• Beneficiário: Raquel Petrilli
• Modalidade de apoio: Bolsas no Brasil - Doutorado