Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment

Full text
Author(s):
Petrillo, R. [1] ; Eloy, J. O. [2] ; Paschoal, J. A. R. [1] ; Lopez, V, R. F.
Total Authors: 4
Affiliation:
[1] V, Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Pharmazie; v. 73, n. 3, p. 133-138, MAR 2018.
Web of Science Citations: 1
Abstract

5-Fluorouracil (5-FU) is used for the topical treatment of pre-cancerous skin lesions. However, previous reports focus on 5-FU quantification in plasma samples, failing to provide information about drug quantification in the skin. Therefore, the aim of this work was to develop a simple and reliable analytical method employing HPLC-UV for 5-FU quantification in skin samples. Chromatographic separation was obtained using the commonly available Lichrospher RP-C18 endcapped column. Porcine ear skin matrix-based analytical curves with thymine, as internal standard, were used. 5-FU was eluted at 5.2 min and thymine at 13.0 min. The method was selective, precise and accurate in the linear range from 0.3 to 6 mu g/mL. The samples were stable after three cycles of freeze/thawing and extraction efficiency rates above 95% were obtained. In vitro skin penetration studies of an aqueous solution and a commercial cream of 5-FU were performed. The cream improved 5-FU retention into the skin and permeation through the skin compared to the solution. Therefore, the method developed herein can be applied to the study of formulations for topical delivery of 5-FU. (AU)

FAPESP's process: 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue
Grantee:Renata Fonseca Vianna Lopez
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/15134-2 - Preparation of immunoliposomes for topical skin cancer treatment
Grantee:Raquel Petrilli
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 12/23764-3 - Topical application of liposomes containing Cetuximab: the effect of physical skin penetration enhancement techniques for cutaneous squamous cell carcinoma
Grantee:Raquel Petrilli
Support Opportunities: Scholarships in Brazil - Doctorate