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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Quantification of 5-FU in skin samples for the development of new delivery systems for topical cancer treatment

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Petrillo, R. [1] ; Eloy, J. O. [2] ; Paschoal, J. A. R. [1] ; Lopez, V, R. F.
Total Authors: 4
[1] V, Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil
[2] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci, Araraquara - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Pharmazie; v. 73, n. 3, p. 133-138, MAR 2018.
Web of Science Citations: 1

5-Fluorouracil (5-FU) is used for the topical treatment of pre-cancerous skin lesions. However, previous reports focus on 5-FU quantification in plasma samples, failing to provide information about drug quantification in the skin. Therefore, the aim of this work was to develop a simple and reliable analytical method employing HPLC-UV for 5-FU quantification in skin samples. Chromatographic separation was obtained using the commonly available Lichrospher RP-C18 endcapped column. Porcine ear skin matrix-based analytical curves with thymine, as internal standard, were used. 5-FU was eluted at 5.2 min and thymine at 13.0 min. The method was selective, precise and accurate in the linear range from 0.3 to 6 mu g/mL. The samples were stable after three cycles of freeze/thawing and extraction efficiency rates above 95% were obtained. In vitro skin penetration studies of an aqueous solution and a commercial cream of 5-FU were performed. The cream improved 5-FU retention into the skin and permeation through the skin compared to the solution. Therefore, the method developed herein can be applied to the study of formulations for topical delivery of 5-FU. (AU)

FAPESP's process: 12/23764-3 - Topical application of liposomes containing cetuximab: the effect of physical skin penetration enhancement techniques for cutaneous squamous cell carcinoma
Grantee:Raquel Petrilli
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/15134-2 - Preparation of immunoliposomes for topical skin cancer treatment
Grantee:Raquel Petrilli
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/22451-7 - Sustained drug delivery systems targeting the epithelial tissue
Grantee:Renata Fonseca Vianna Lopez
Support type: Research Projects - Thematic Grants