Improvement of cutaneous delivery of methylene blue by liquid crystals

Autor(es)

Junqueira Garcia, Maria Teresa ^[1] ; Goncalves, Thalita Pedralino ^[1] ; Martins, Ericka Sao Felix ^[1] ; Martins, Tereza Silva ^[1] ; de Abreu Fantini, Marcia Carvalho ^[2] ; Regazi Minarini, Paulo Roberto ^[1] ; Fernandez, Sandra Costa ^[3] ; Salata, Giovanna Cassone ^[3] ; Lopes, Luciana Biagini ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Rua Sao Nicolau 210, Diadema, SP - Brazil

^[2] Univ Sao Paulo, Inst Fis, Rua Matao 1371, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Av Prof Lineu Prestes 1524, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Pharmaceutics; v. 548, n. 1, p. 454-465, SEP 2018.
• Citações Web of Science: 5

Resumo

The purpose of this study was to evaluate the effect of composition and characteristics of liquid crystalline phases (LCPs) on cutaneous delivery of methylene blue (MB). LCPs were obtained by mixing Brij97 (R) with water at various ratios; Brij97 (R) : water at 8:2 (F8:2), 7:3 (F7:3), and 6:4 (F6:4) were selected, and MB was incorporated at 0.1%. F8:2 and F7:3 exhibited textures and small angle X-ray scattering (SAXS) patterns corresponding to lamellar phase, whereas F6:4 displayed characteristics of hexagonal phase. All three LCPs were stable for 9 months, and exhibited thixotropic pseudoplastic behaviour. Increasing water content increased viscosity. All three LCPs released less (3.2- to 6.6-fold) MB than control gel (3.0% hydroxyethylcellulose (HEC) + 0.1% MB), demonstrating their ability to sustain release. Despite the lower release, all LCPs improved skin retention of MB at 6 h post-application (1.3- to 2.1-fold) compared to the control gel. Among the LCPs, F8:2-mediated skin retention of MB was more pronounced, followed by F7:3. Consistent with the increased penetration, transepidermal water loss (TEWL) also increased after treatment with the LCPs (2.0-2.8 fold), which suggests their influence on skin barrier. Irritation studies by Hen's Egg Test - Chorioallantoic Membrane (HET-CAM) suggest that F7:3 and F6:4 may be better tolerated by the skin than F8:2. (AU)

• Processo FAPESP: 13/16617-7 - Sistemas nanoestruturados tópicos para co-localização de agentes quimioterápicos e quimiopreventivos na pele e mama
• Beneficiário: Luciana Biagini Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/23213-0 - Sistemas biorresponsivos para liberação sustentada de fármacos visando a quimioprevenção do câncer de mama
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• Modalidade de apoio: Bolsas no Brasil - Mestrado
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• Beneficiário: Richardt Gama Landgraf
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores