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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Making a Better Home: Modulation of Plant Defensive Response by Brevipalpus Mites

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Autor(es):
Arena, Gabriella D. [1, 2] ; Ramos-Gonzalez, Pedro L. [3] ; Rogerio, Luana A. [2] ; Ribeiro-Alves, Marcelo [4] ; Casteel, Clare L. [5] ; Freitas-Astua, Juliana [3, 6] ; Machado, Marcos A. [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil
[2] Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, Lab Biotecnol, Cordeiropolis - Brazil
[3] Inst Biol, Lab Bioquim Fitopatol, Sao Paulo - Brazil
[4] Fundacao Oswaldo Cruz, Inst Nacl Infectol Evandro Chagas, Rio De Janeiro - Brazil
[5] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 - USA
[6] Embrapa Mandioca & Fruticultura, Cruz Das Almas - Brazil
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PLANT SCIENCE; v. 9, AUG 15 2018.
Citações Web of Science: 4
Resumo

False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growth-defense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the non-infested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA-or JA-mediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway. (AU)

Processo FAPESP: 17/50222-0 - Compreensão sobre a biologia molecular e ecologia da relações planta-vírus-vetor: rumo a estratégias sustentáveis e integradas de manejo de vírus
Beneficiário:Juliana de Freitas Astúa
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/00366-8 - Arabidopsis thaliana e Citrus leprosis virus-C: um modelo para o estudo da interação planta-patógeno
Beneficiário:Gabriella Dias Arena
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros
Beneficiário:Marcos Antonio Machado
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/23870-9 - Infecção por Cilevirus: uma abordagem para a interação entre as proteínas do vírus e da planta
Beneficiário:Gabriella Dias Arena
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Doutorado