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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Making a Better Home: Modulation of Plant Defensive Response by Brevipalpus Mites

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Author(s):
Arena, Gabriella D. [1, 2] ; Ramos-Gonzalez, Pedro L. [3] ; Rogerio, Luana A. [2] ; Ribeiro-Alves, Marcelo [4] ; Casteel, Clare L. [5] ; Freitas-Astua, Juliana [3, 6] ; Machado, Marcos A. [2]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Campinas, SP - Brazil
[2] Inst Agron Campinas, Ctr Citricultura Sylvio Moreira, Lab Biotecnol, Cordeiropolis - Brazil
[3] Inst Biol, Lab Bioquim Fitopatol, Sao Paulo - Brazil
[4] Fundacao Oswaldo Cruz, Inst Nacl Infectol Evandro Chagas, Rio De Janeiro - Brazil
[5] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 - USA
[6] Embrapa Mandioca & Fruticultura, Cruz Das Almas - Brazil
Total Affiliations: 6
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 9, AUG 15 2018.
Web of Science Citations: 4
Abstract

False-spider mites of the genus Brevipalpus are highly polyphagous pests that attack hundreds of plant species of distinct families worldwide. Besides causing direct damage, these mites may also act as vectors of many plant viruses that threaten high-value ornamental plants like orchids and economically important crops such as citrus and coffee. To better understand the molecular mechanisms behind plant-mite interaction we used an RNA-Seq approach to assess the global response of Arabidopsis thaliana (Arabidopsis) plants along the course of the infestation with Brevipalpus yothersi, the main vector species within the genus. Mite infestation triggered a drastic transcriptome reprogramming soon at the beginning of the interaction and throughout the time course, deregulating 1755, 3069 and 2680 genes at 6 hours after infestation (hai), 2 days after infestation (dai), and 6 dai, respectively. Gene set enrichment analysis revealed a clear modulation of processes related to the plant immune system. Co-expressed genes correlated with specific classes of transcription factors regulating defense pathways and developmental processes. Up-regulation of defensive responses correlated with the down-regulation of growth-related processes, suggesting the triggering of the growth-defense crosstalk to optimize plant fitness. Biological processes (BPs) enriched at all time points were markedly related to defense against herbivores and other biotic stresses involving the defense hormones salicylic acid (SA) and jasmonic acid (JA). Levels of both hormones were higher in plants challenged with mites than in the non-infested ones, supporting the simultaneous induction of genes from both pathways. To further clarify the functional relevance of the plant hormonal pathways on the interaction, we evaluated the mite performance on Arabidopsis mutants impaired in SA-or JA-mediated response. Mite oviposition was lower on mutants defective in SA biosynthesis (sid2) and signaling (npr1), showing a function for SA pathway in improving the mite reproduction, an unusual mechanism compared to closely-related spider mites. Here we provide the first report on the global and dynamic plant transcriptome triggered by Brevipalpus feeding, extending our knowledge on plant-mite interaction. Furthermore, our results suggest that Brevipalpus mites manipulate the plant defensive response to render the plant more susceptible to their colonization by inducing the SA-mediated pathway. (AU)

FAPESP's process: 17/50222-0 - Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies
Grantee:Juliana de Freitas Astúa
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50880-0 - INCT 2014: comparative and functional genomics and citrus-assisted breeding
Grantee:Marcos Antonio Machado
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/00366-8 - Arabidopsis thaliana e Citrus leprosis virus-C: a model for the plant-pathogen interaction study
Grantee:Gabriella Dias Arena
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 16/23870-9 - Cilevirus infection: An approach to the viral-plant protein interplay
Grantee:Gabriella Dias Arena
Support Opportunities: Scholarships abroad - Research Internship - Doctorate