Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor

Autor(es)

Datilo, Marcella Neves ^{[1, 2]} ; Sant'Ana, Marcella Ramos ^{[1, 2]} ; Formigari, Guilherme Pedron ^[1] ; Rodrigues, Patricia Brito ^{[1, 2]} ; de Moura, Leandro Pereira ^[3] ; Ramos da Silva, Adelino Sanchez ^[4] ; Ropelle, Eduardo Rochete ^[3] ; Pauli, Jose Rodrigo ^[3] ; Cintra, Dennys Esper ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Appl Sci, LabGeN, Nutr Genom Lab, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci, CELN, Nutrigenom & Lipids Res Ctr, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Sch Appl Sci, LabMEx, Lab Mol Biol Exercise, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 8, SEP 25 2018.
• Citações Web of Science: 6

Resumo

The chronic and low-grade inflammation induced by obesity seem to be the ``first hit{''} to retinopathy associated to diabetes type 2. Herein, we hypothesized that omega-3 fatty acids from flaxseed oil enriched diet disrupt the pro-inflammatory status in the retina, protecting against retinopathy development. For eight weeks under a high-fat diet (HF), several physiological parameters were monitored to follow the metabolic homeostasis disruption. After this period, mice were treated with a HF substituted in part of lard by flaxseed oil (FS) for another eight weeks. Food behavior, weight gain, glucose and insulin sensitivity, electroretinography, RT-qPCR and western blots were carried out. The HF was able to induce a pro-inflammatory background in the retina, changing IL1 beta and TNF alpha. VEGF, a master piece of retinopathy, had early onset increased also induced by HF. The FS-diet was able to decrease inflammation and retinopathy and improved retinal electro stimuli compared to HF group. GPR120 and GPR40 (G Protein-Coupled Receptors 120 and 40), an omega-3 fatty acid receptors, were detected in the retina for the first time. FS-diet modulated the gene expression and protein content of these receptors. Thus, unsaturated fatty acids protect the retina from diabetes type 2 mice model from disease progression. (AU)

• Processo FAPESP: 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas
• Beneficiário: Licio Augusto Velloso
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/07129-6 - Ácidos graxos W3 e W9 no bloqueio de vias inflamatórias através do receptor GPR120: uma abordagem multi-orgânica
• Beneficiário: Dennys Esper Corrêa Cintra
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores