| Full text | |
| Author(s): |
Datilo, Marcella Neves
[1, 2]
;
Sant'Ana, Marcella Ramos
[1, 2]
;
Formigari, Guilherme Pedron
[1]
;
Rodrigues, Patricia Brito
[1, 2]
;
de Moura, Leandro Pereira
[3]
;
Ramos da Silva, Adelino Sanchez
[4]
;
Ropelle, Eduardo Rochete
[3]
;
Pauli, Jose Rodrigo
[3]
;
Cintra, Dennys Esper
[1, 2]
Total Authors: 9
|
| Affiliation: | [1] Univ Estadual Campinas, Sch Appl Sci, LabGeN, Nutr Genom Lab, Sao Paulo - Brazil
[2] Univ Estadual Campinas, Sch Appl Sci, CELN, Nutrigenom & Lipids Res Ctr, Sao Paulo - Brazil
[3] Univ Estadual Campinas, Sch Appl Sci, LabMEx, Lab Mol Biol Exercise, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 8, SEP 25 2018. |
| Web of Science Citations: | 6 |
| Abstract | |
The chronic and low-grade inflammation induced by obesity seem to be the ``first hit{''} to retinopathy associated to diabetes type 2. Herein, we hypothesized that omega-3 fatty acids from flaxseed oil enriched diet disrupt the pro-inflammatory status in the retina, protecting against retinopathy development. For eight weeks under a high-fat diet (HF), several physiological parameters were monitored to follow the metabolic homeostasis disruption. After this period, mice were treated with a HF substituted in part of lard by flaxseed oil (FS) for another eight weeks. Food behavior, weight gain, glucose and insulin sensitivity, electroretinography, RT-qPCR and western blots were carried out. The HF was able to induce a pro-inflammatory background in the retina, changing IL1 beta and TNF alpha. VEGF, a master piece of retinopathy, had early onset increased also induced by HF. The FS-diet was able to decrease inflammation and retinopathy and improved retinal electro stimuli compared to HF group. GPR120 and GPR40 (G Protein-Coupled Receptors 120 and 40), an omega-3 fatty acid receptors, were detected in the retina for the first time. FS-diet modulated the gene expression and protein content of these receptors. Thus, unsaturated fatty acids protect the retina from diabetes type 2 mice model from disease progression. (AU) | |
| FAPESP's process: | 13/07607-8 - OCRC - Obesity and Comorbidities Research Center |
| Grantee: | Licio Augusto Velloso |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 12/07129-6 - W3 and W9 fatty acids as inflammatory pathway blockers through GPR120 receptor: a multi-organic approach |
| Grantee: | Dennys Esper Corrêa Cintra |
| Support Opportunities: | Research Grants - Young Investigators Grants |