BGP-15 improves contractile function of regenerating soleus muscle

Autor(es)

Nascimento, Tabata L. ^[1] ; Silva, Meiricris T. ^[1] ; Miyabara, Elen H. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Av Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY; v. 39, n. 1-2, p. 25-34, APR 2018.
• Citações Web of Science: 1

Resumo

This study investigated the effect of the heat shock protein inducer O-{[}3-piperidino-2-hydroxy-1-propyl]-nicotinic amidoxime (BGP-15) on the morphology and contractile function of regenerating soleus muscles from mice. Cryolesioned soleus muscles from young mice treated daily with BGP-15 (15mg/Kg) were evaluated on post-cryolesion day 10. At this time point, there was a significant decrease in the cross-sectional area of regenerating myofibers, maximal force, specific tetanic force, and fatigue resistance of regenerating soleus muscles. BGP-15 did not reverse the decrease in myofiber cross-sectional area but effectively prevented the reduction in tetanic force and fatigue resistance of regenerating muscles. In addition, BGP-15 treatment increased the expression of embryonic myosin heavy chain (e-MyHC), MyHC-II and MyHC-I in regenerating muscles. Although BGP-15 did not alter voltage dependent anion-selective channel 2 (VDAC2) expression in cryolesioned muscles, it was able to increase inducible 70-kDaheat shock protein(HSP70) expression. Our results suggest that BGP-15 improves strength recovery in regenerating soleus muscles by accelerating the re-expression of adult MyHC-II and MyHC-I isoforms and HSP70 induction. The beneficial effects of BGP-15 on the contractile function of regenerating muscles reinforce the potential of this molecule to be used as a therapeutic agent. (AU)

• Processo FAPESP: 14/13874-1 - Regeneração muscular esquelética de camundongos com Síndrome de Marfan: estudo do potencial regenerativo das células satélites
• Beneficiário: Meiricris Tomaz da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/23391-8 - Resposta regenerativa da musculatura esquelética de camundongos com síndrome de Marfan
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/04783-0 - Influência das proteínas de choque térmico na resposta regenerativa de músculos esqueléticos de camundongos idosos
• Beneficiário: Tábata Leal Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado