| Full text | |
| Author(s): |
Total Authors: 3
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Av Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 1
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| Document type: | Journal article |
| Source: | JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY; v. 39, n. 1-2, p. 25-34, APR 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
This study investigated the effect of the heat shock protein inducer O-{[}3-piperidino-2-hydroxy-1-propyl]-nicotinic amidoxime (BGP-15) on the morphology and contractile function of regenerating soleus muscles from mice. Cryolesioned soleus muscles from young mice treated daily with BGP-15 (15mg/Kg) were evaluated on post-cryolesion day 10. At this time point, there was a significant decrease in the cross-sectional area of regenerating myofibers, maximal force, specific tetanic force, and fatigue resistance of regenerating soleus muscles. BGP-15 did not reverse the decrease in myofiber cross-sectional area but effectively prevented the reduction in tetanic force and fatigue resistance of regenerating muscles. In addition, BGP-15 treatment increased the expression of embryonic myosin heavy chain (e-MyHC), MyHC-II and MyHC-I in regenerating muscles. Although BGP-15 did not alter voltage dependent anion-selective channel 2 (VDAC2) expression in cryolesioned muscles, it was able to increase inducible 70-kDaheat shock protein(HSP70) expression. Our results suggest that BGP-15 improves strength recovery in regenerating soleus muscles by accelerating the re-expression of adult MyHC-II and MyHC-I isoforms and HSP70 induction. The beneficial effects of BGP-15 on the contractile function of regenerating muscles reinforce the potential of this molecule to be used as a therapeutic agent. (AU) | |
| FAPESP's process: | 14/13874-1 - Skeletal muscle regeneration in mice with Marfan Syndrome: study of the regenerative potential of satellite cells |
| Grantee: | Meiricris Tomaz da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/23391-8 - Regenerative response of skeletal muscles from mice with Marfan Syndrome |
| Grantee: | Elen Haruka Miyabara |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/04783-0 - The influence of heat shock proteins in the recovery of skeletal muscle of old mice |
| Grantee: | Tábata Leal Nascimento |
| Support Opportunities: | Scholarships in Brazil - Doctorate |