Exercise activates the hypothalamic S1PR1-STAT3 axis through the central action of interleukin 6 in mice

Autor(es):Mostrar menos -

Silva, Vagner R. R. ^{[1, 2]} ; Micheletti, Thayana O. ^[1] ; Katashima, Carlos K. ^[1] ; Lenhare, Luciene ^{[1, 2]} ; Morari, Joseane ^[3] ; Moura-Assis, Alexandre ^[3] ; de Lima-Junior, Jose C. ^[3] ; Camargo, Juliana A. ^[1] ; Passos, Gabriela R. ^[1] ; Gaspar, Rodrigo S. ^[2] ; Velloso, Licio A. ^[3] ; Saad, Mario J. ^[1] ; da Silva, Adelino S. R. ^{[4, 5, 6]} ; Moura, Leandro P. ^[2] ; Cintra, Dennys E. ^[5] ; Pauli, Jose R. ^{[2, 7]} ; Ropelle, Eduardo R. ^{[1, 2, 7, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, BR-13484350 Limeira, SP - Brazil

^[3] Univ Estadual Campinas, Obes & Comorbid Res Ctr OCRC, Lab Cell Signaling, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Campinas, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP - Brazil

^[6] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil

^[7] Univ Estadual Campinas, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Limeira, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 233, n. 12, p. 9426-9436, DEC 2018.
• Citações Web of Science: 0

Resumo

Hypothalamic sphingosine-1-phosphate receptor 1 (S1PR1), the G protein-coupled receptor 1 of sphingosine-1-phosphate, has been described as a modulator in the control of energy homeostasis in rodents. However, this mechanism is still unclear. Here, we evaluate the role of interleukin 6 (IL-6) associated with acute physical exercise in the control of the hypothalamic S1PR1-signal transducer and activator of transcription 3 (STAT3) axis. Acute exercise session and an intracerebroventricular IL-6 injection increased S1PR1 protein content and STAT3 phosphorylation in the hypothalamus of lean and obese mice accompanied by a reduction in food consumption. Transcriptome analysis indicated a strong positive correlation between Il-6 and S1pr1 messenger RNA in several tissues of genetically diverse BXD mice strains and humans, including in the hypothalamus. Interestingly, exercise failed to stimulate the S1PR1-STAT3 axis in IL-6 knockout mice and the disruption of hypothalamic-specific IL-6 action blocked the anorexigenic effects of exercise. Taken together, our results indicate that physical exercise modulates the S1PR1 protein content in the hypothalamus, through the central action of IL-6. (AU)

• Processo FAPESP: 16/01089-3 - Avaliação da UPRmt e do desequilíbrio mitonuclear em resposta ao treinamento físico: Uma abordagem multi-orgânica.
• Beneficiário: Eduardo Rochete Ropelle
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/20052-5 - Os efeitos do exercício físico sobre a sensibilidade a glicose em hipotálamo de ratos: o papel da proteína HIF2 alfa.
• Beneficiário: Vagner Ramon Rodrigues Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/09656-0 - Caracterização da proteína S1PR1 hipotalâmica no controle da ingestão alimentar em roedores
• Beneficiário: Eduardo Rochete Ropelle
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores