Effect of swimming training on nerve morphological recovery after compressive injury

Autor(es)

de Moraes, Alexa Alves ^[1] ; Soares de Almeida, Cayo Antonio ^[2] ; Lucas, Guilherme ^[3] ; Thomazini, Jose Antonio ^[4] ; DeMaman, Aline Santos ^[5]

Afiliação do(s) autor(es)

^[1] Paraiba State Univ, Ctr Biol & Hlth Sci, Dept Physiotherapy, Campina Grande - Brazil

^[2] Fed Univ ABC, Ctr Math Comp Sci & Cognit, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Surg & Anat, Ribeirao Preto - Brazil

^[5] Paraiba State Univ, Ctr Biol & Hlth Sci, Dept Biol, Campina Grande - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NEUROLOGICAL RESEARCH; v. 40, n. 11, p. 955-962, NOV 2 2018.
• Citações Web of Science: 0

Resumo

Objective: This study aims to investigate morphological alterations caused by partial sciatic nerve ligation (PNL) and the efficacy of a moderate-intensity swimming training as therapeutic strategy for nerve regeneration. Methods: A number of 30 male adult mice were equally divided in control, 14 days after PNL (PNL 14 days), 42 days after PNL (PNL 42 days), 70 days after PNL (PNL 70 days) and 5-week exercise training after 7 days post-lesion (PNL trained 35 days) groups. PNL trained 35 days group began with a 10-min session for 3 days and this time was gradually increased by 10 min every three sessions until the animals had swum for 50 min per session. Morphoquantitative analysis was carried out to assess nerve regeneration in each group. Results: PNL 14 days group exhibited less degenerating signs than PNL 42 days group, where most post-lesion alterations were visualized. Nerve area and minimum diameter were significantly lower (p < 0.05) than control group. PNL 70 days group showed a greater degree of regenerating fibers and similar morphometric parameters to control group. PNL trained 35 days demonstrated signs of regeneration, reaching control group values in the morphometric analysis. Discussion: PNL promotes great histopathological changes, which became more visible at 42 post-injury days. A natural nerve-regeneration tendency was observed throughout time, as observed in PNL 70 days group; nevertheless, moderate swimming training was found to be a therapeutic resource for nerve regeneration, accelerating such process from a morphoquantitative perspective. Abbreviations: ANOVA: One-way analysis of variance; BDNF: Brain-derived neurotrophic factor; FGF-2: Fibroblast growth factor 2; GDNF: Glial cell line derived neurotrophic factor; IGF: Insulin-link growth factor; IL-1 beta: Interleukin-1 beta; NGF: Neural growth factor; PBS: Phosphate-buffered saline; PNL: Partial sciatic nerve ligation (AU)

• Processo FAPESP: 11/08364-6 - Ação do exercício sobre a expressão e atividade de ligantes da família do Fator Neurotrófico Derivado da Glia: integrando mecanismos de dor neuropática e o valor terapêutico da atividade física.
• Beneficiário: Aline dos Santos de Maman
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado