Permeability of DOPC bilayers under photoinduced oxidation: Sensitivity to photosensitizer

Autor(es)

Bacellar, Isabel O. L. ^{[1, 2]} ; Baptista, Mauricio S. ^[1] ; Junqueira, Helena C. ^[1] ; Wainwright, Mark ^[3] ; Thalmann, Fabrice ^[2] ; Marques, Carlos M. ^[2] ; Schroder, Andre P. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil

^[2] Univ Strasbourg, Inst Charles Sadron, CNRS, UP22, 23 Rue Loess, F-67200 Strasbourg - France

^[3] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside - England

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES; v. 1860, n. 11, p. 2366-2373, NOV 2018.
• Citações Web of Science: 5

Resumo

The modification of lipid bilayer permeability is one of the most striking yet poorly understood physical transformations that follow photoinduced lipid oxidation. We have recently proposed that the increase of permeability of photooxidized 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayers is controlled by the time required by the oxidized lipid species to diffuse and aggregate into pores. Here we further probe this mechanism by studying photosensitization of DOPC membranes by methylene blue (MB) and DO15, a more hydrophobic phenothiazinium photosensitizer, under different irradiation powers. Our results not only reveal the interplay between the production rate and the diffusion of the oxidized lipids, but highlight also the importance of photosensitizer localization in the kinetics of oxidized membrane permeability. (AU)

• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/11640-0 - Relação entre o dano fotoinduzido em lipídeos e a permeabilização de membranas
• Beneficiário: Isabel de Oliveira Lima Bacellar
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto