Diniz, Giovanne B.
Candido, Paulo L.
Klein, Marianne O.
Alvisi, Renato D.
[4, 5, 6]
Felicio, Luciano F.
Bittencourt, Jackson C.
Número total de Autores: 8
Afiliação do(s) autor(es):
 Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Chem Neuroanat, BR-05508000 Sao Paulo - Brazil
 Santa Marcelina Med Sch, Dept Anat, Sao Paulo - Brazil
 Univ Sao Paulo, Sch Vet Med, Dept Pathol, Sao Paulo - Brazil
 Inst Pharmacol Mol & Cellulaire, CNRS UNS UMR 7275, Valbonne - France
 Univ Cote dAzur, Nice - France
 Stn Primatol UPS846 CNRS, Rousset Sur Arc - France
 Univ Sao Paulo, Inst Psychol, Ctr Neurosci & Behav, Sao Paulo - Brazil
Número total de Afiliações: 7
Tipo de documento:
Brain Structure & Function;
Citações Web of Science:
The orexin-immunoreactive neurons are part of an important arousal-promoting hypothalamic population. Several groups have investigated these neurons during the lactation period, when numerous physiological alterations occur in the dam's body to cope with the newly acquired metabolic needs of the litter. Although those studies have probed this population during the early and intermediate stages of lactation, few works have examined its response to weaning, including the cessation of the tactile suckling stimulus as the litter stops nursing. Using double immunohistochemistry for orexin and FOS combined with three-dimensional reconstruction techniques, we investigated orexin-synthesizing neurons and their activation at different times during weaning, in addition to the role played by the suckling stimulus. We report here that weaning promoted a decline in the anterior population of orexin-immunoreactive neurons and decreased the number of double orexin-FOS neurons labeled in the central dorsomedial hypothalamus, in addition to reducing the overall number of FOS-immunoreactive cells in the whole tuberal hypothalamus. Disruption of the suckling stimulus from the pups impaired the decrease in the number of anteriorly located orexin-immunoreactive neurons, attenuated the activation of orexin-synthesizing cells in the dorsomedial hypothalamus and reduced the number of FOS-immunoreactive neurons across the tuberal hypothalamus. When taken together, our data suggest that the weaning period is necessary to restore neurochemical pathways altered during the lactation period and that the suckling stimulus plays a significant role in this process. (AU)