The weaning period promotes alterations in the orexin neuronal population of rats in a suckling-dependent manner

Autor(es)

Diniz, Giovanne B. ^[1] ; Candido, Paulo L. ^{[1, 2]} ; Klein, Marianne O. ^[1] ; Alvisi, Renato D. ^[3] ; Presse, Francoise ^{[4, 5]} ; Nahon, Jean-Louis ^{[4, 5, 6]} ; Felicio, Luciano F. ^[3] ; Bittencourt, Jackson C. ^{[1, 7]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Chem Neuroanat, BR-05508000 Sao Paulo - Brazil

^[2] Santa Marcelina Med Sch, Dept Anat, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Sao Paulo - Brazil

^[4] Inst Pharmacol Mol & Cellulaire, CNRS UNS UMR 7275, Valbonne - France

^[5] Univ Cote dAzur, Nice - France

^[6] Stn Primatol UPS846 CNRS, Rousset Sur Arc - France

^[7] Univ Sao Paulo, Inst Psychol, Ctr Neurosci & Behav, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brain Structure & Function; v. 223, n. 8, p. 3739-3755, NOV 2018.
• Citações Web of Science: 1

Resumo

The orexin-immunoreactive neurons are part of an important arousal-promoting hypothalamic population. Several groups have investigated these neurons during the lactation period, when numerous physiological alterations occur in the dam's body to cope with the newly acquired metabolic needs of the litter. Although those studies have probed this population during the early and intermediate stages of lactation, few works have examined its response to weaning, including the cessation of the tactile suckling stimulus as the litter stops nursing. Using double immunohistochemistry for orexin and FOS combined with three-dimensional reconstruction techniques, we investigated orexin-synthesizing neurons and their activation at different times during weaning, in addition to the role played by the suckling stimulus. We report here that weaning promoted a decline in the anterior population of orexin-immunoreactive neurons and decreased the number of double orexin-FOS neurons labeled in the central dorsomedial hypothalamus, in addition to reducing the overall number of FOS-immunoreactive cells in the whole tuberal hypothalamus. Disruption of the suckling stimulus from the pups impaired the decrease in the number of anteriorly located orexin-immunoreactive neurons, attenuated the activation of orexin-synthesizing cells in the dorsomedial hypothalamus and reduced the number of FOS-immunoreactive neurons across the tuberal hypothalamus. When taken together, our data suggest that the weaning period is necessary to restore neurochemical pathways altered during the lactation period and that the suckling stimulus plays a significant role in this process. (AU)

• Processo FAPESP: 17/08908-2 - O Hormônio Concentrador de Melanina como um Peptídeo Integrativo Promovendo Comportamentos Motivados
• Beneficiário: Jackson Cioni Bittencourt
• Linha de fomento: Auxílio à Pesquisa - Publicações científicas - Artigo
• Processo FAPESP: 16/02748-0 - Estudo das bases anatômicas para um mecanismo de transmissão de volume no sistema peptidérgico do hormônio concentrador de melanina [MCH]
• Beneficiário: Giovanne Baroni Diniz
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/02224-1 - A existência da neurogênese durante o período da lactação
• Beneficiário: Jackson Cioni Bittencourt
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/52068-0 - A participação do hormônio concentrador de melanina no controle da lactação
• Beneficiário: Jackson Cioni Bittencourt
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/17998-5 - Avaliação dos efeitos da administração crônica do hormônio concentrador de melanina (MCH) em ratas Long-Evans lactantes
• Beneficiário: Marianne Orlandini Klein
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado