Naringenin mitigates titanium dioxide (TiO2)-induced chronic arthritis in mice: role of oxidative stress, cytokines, and NF kappa B

Autor(es):Mostrar menos -

Manchope, Marilia F. ^[1] ; Artero, Nayara A. ^[2] ; Fattori, Victor ^[1] ; Mizokami, Sandra S. ^[1] ; Pitol, Dimitrius L. ^[3] ; Issa, Joao P. M. ^[3] ; Fukada, Sandra Y. ^[4] ; Cunha, Thiago M. ^[5] ; Alves-Filho, Jose C. ^[5] ; Cunha, Fernando Q. ^[5] ; Casagrande, Rubia ^[2] ; Verri, Jr., Waldiceu A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Ciencias Patol, Rod Celso Garcia Cid Km480 PR445, Cx Postal 10-011, BR-86057970 Londrina, Parana - Brazil

^[2] Univ Estadual Londrina, Ctr Ciencias Saude, Dept Ciencias Farmaceut, Londrina - Brazil

^[3] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Fisiol & Patol Basica, Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Fis & Quim, Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Farmacol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Inflammation Research; v. 67, n. 11-12, p. 997-1012, DEC 2018.
• Citações Web of Science: 2

Resumo

ObjectiveTo evaluate the effect and mechanisms of naringenin in TiO2-induced chronic arthritis in mice, a model resembling prosthesis and implant inflammation.TreatmentFlavonoids are antioxidant and anti-inflammatory molecules with important anti-inflammatory effect. Mice were daily treated with the flavonoid naringenin (16.7-150mg/kg, orally) for 30 days starting 24h after intra-articular knee injection of 3mg of TiO2.MethodsTiO(2)-induced arthritis resembles cases of aseptic inflammation induced by prosthesis and/or implants. Mice were stimulated with 3mg of TiO2 and after 24h mice started to be treated with naringenin. The disease phenotype, treatment toxicity, histopathological damage, oxidative stress, cytokine expression and NFB were evaluated after 30 days of treatment.ResultsNaringenin inhibited TiO2-induced mechanical hyperalgesia (96%), edema (77%) and leukocyte recruitment (74%) without inducing toxicity. Naringenin inhibited histopathological index (HE, 49%), cartilage damage (Toluidine blue tibial staining 49%, and proteoglycan 98%), and bone resorption (TRAP-stained 73%). These effects were accompanied by inhibition of oxidative stress (gp91(phox) 93%, NBT 83%, and TBARS 41%) cytokine mRNA expression (IL-33 82%, TNF 76%, pro-IL-1 100%, and IL-6 61%), and NFB activation (100%).ConclusionNaringenin ameliorates TiO2-induced chronic arthritis inducing analgesic and anti-inflammatory responses with improvement in the histopathological index, cartilage damage, and bone resorption. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/19670-0 - Mecanismos envolvidos na fisiopatologia da artrite reumatóide, dor e sepse
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Temático