A comparative venomic fingerprinting approach reveals that galling and non-galling fig wasp species have different venom profiles

Autor(es)

Elias, Larissa G. ^[1] ; Silva, Denise B. ^{[2, 3]} ; Silva, Ricardo ^[2] ; Peng, Yan-Qiong ^[4] ; Yang, Da-Rong ^[4] ; Lopes, Norberto P. ^[2] ; Pereira, Rodrigo A. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, NPPNS, Fac Ciencias Farmaceut Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[3] Univ Fed Mato Grosso do Sul, Lab Prod Nat & Espectrometria Massas LaPNEM, Campo Grande, MS - Brazil

^[4] Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Mengla, Yunnan - Peoples R China

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 11 NOV 8 2018.
• Citações Web of Science: 1

Resumo

The galling habit represents a complex type of interaction between insects and plants, ranging from antagonism to mutualism. The obligate pollination mutualism between Ficus and fig wasps relies strongly on the induction of galls in Ficus flowers, where wasps' offspring develop. Even though gall induction plays an important role in many insect-plant interactions, the mechanisms that trigger gall formation are still not completely known. Using a fingerprinting approach, we show here that venom protein profiles from galling fig wasps differ from the venom profiles of non-galling species, suggesting the secretion plays different roles according to the type of interaction it is involved in. Each studied cleptoparasitic species had a distinct venom profile, suggesting that cleptoparasitism in fig wasps covers a vast diversity of molecular interactions. Fig wasp venoms are mainly composed of peptides. No low molecular weight compounds were detected by UPLC-DAD-MS, suggesting that such compounds (e.g., IAA and cytokinines) are not involved in gall induction. The differences in venom composition observed between galling and non-galling fig wasp species bring new perspectives to the study of gall induction processes and the role of insect secretions. (AU)

• Processo FAPESP: 14/01884-2 - Análise de perfis de metabólitos obtidos por espectrometria de massas utilizando grafos
• Beneficiário: Ricardo Roberto da Silva
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/01918-1 - Papel das glândulas de veneno na biologia larval em espécies de vespas de figo (Hymenoptera: Chalcidoidea)
• Beneficiário: Larissa Galante Elias
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50265-3 - Metabolismo e distribuição de xenobióticos naturais e sintéticos: da compreensão dos processos reacionais à geração de imagens teciduais
• Beneficiário: Norberto Peporine Lopes
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático