Transcriptome of the Wistar audiogenic rat (WAR) strain following audiogenic seizures

Autor(es)

Damasceno, Samara ^[1] ; de Menezes, Nadialia Bustamante ^[1] ; Rocha, Cristiane de Souza ^[2] ; Berenguer de Matos, Alexandre Hilario ^[2] ; Vieira, Andre Schwambach ^[2] ; Dutra Moraes, Marcio Flavio ^[3] ; Martins, Almir Souza ^[3] ; Lopes-Cendes, Iscia ^[2] ; Brunialti Godard, Ana Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Minas Gerais, Dept Biol Geral, BR-31270901 Belo Horizonte, MG - Brazil

^[2] Univ Estadual Campinas, Fac Ciencias Med, Dept Genet Med, Cidade Univ Zeferino Vaz, BR-13083887 Campinas, SP - Brazil

^[3] Univ Fed Minas Gerais, Dept Fisiol & Biofis, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Epilepsy Research; v. 147, p. 22-31, NOV 2018.
• Citações Web of Science: 0

Resumo

The Wistar Audiogenic Rat (WAR) is a model whose rats are predisposed to develop seizures following acoustic stimulation. We aimed to establish the transcriptional profile of the WAR model, searching for genes that help in understanding the molecular mechanisms involved in the predisposition and seizures expression of this strain. RNA-Seq of the corpora quadrigemina of WAR and Wistar rats subjected to acoustic stimulation revealed 64 genes differentially regulated in WAR. We validated twelve of these genes by qPCR in stimulated and naive (non stimulated) WAR and Wistar rats. Among these, Acsm3 was upregulated in WAR in comparison with both control groups. In contrast, Gpr126 and Rteli were downregulated in naive and stimulated WAR rats in comparison with the Wistar controls. Qdpr was upregulated only in stimulated WAR rats that exhibited audiogenic seizures. Our data show that there are genes with differential intrinsic regulation in the WAR model and that seizures can alter gene regulation. We identified new genes that might be involved in the epileptic phenotype and comorbidities of the WAR model. (AU)

• Processo FAPESP: 09/53444-8 - Estudo da expressão gênica em larga escala e análise funcional utilizando a tecnologia da interferência por RNA (RNAi) em um modelo de epilepsia audiogênica em ratos
• Beneficiário: Iscia Teresinha Lopes Cendes
• Modalidade de apoio: Auxílio à Pesquisa - Regular