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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Electron Paramagnetic Resonance and Small-Angle X-ray Scattering Characterization of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers for Dibucaine Encapsulation

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Autor(es):
Barbosa, Raquel M. [1, 2, 3] ; Casadei, Bruna R. [4] ; Duarte, Evandro L. [5] ; Severino, Patricia [6, 7] ; Barbosa, Leandro R. S. [5] ; Duran, Nelson ; de Paula, Eneida [2]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Campinas Unicamp, Inst Chem, BR-13083861 Campinas, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Biochem & Tissue Biol Dept, BR-13083862 Campinas, SP - Brazil
[3] UNINASSAU Coll, Pharm Dept, BR-59080400 Natal, RN - Brazil
[4] Fed Univ Sao Paulo UNIFESP, Biophys Dept, BR-04021001 Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Phys Inst, BR-05508090 Sao Paulo, SP - Brazil
[6] Tiradentes Univ UNIT, Lab Nanotechnol & Nanomed LNMED, Ave Murilo Dantas, 300, BR-49010390 Aracaju, Sergipe - Brazil
[7] Inst Technol & Res ITP, Ave Murilo Dantas, 300, BR-49010390 Aracaju, Sergipe - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: Langmuir; v. 34, n. 44, p. 13296-13304, NOV 6 2018.
Citações Web of Science: 6
Resumo

Dibucaine (DBC) is one of the most potent long-acting local anesthetics, but it also has significant toxic side effects and low water solubility. Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) have been proposed as drug-delivery systems to increase the bioavailability of local anesthetics. The purpose of the present study was to characterize SLNs and NLCs composed of cetyl palmitate or myristyl myristate, a mixture of capric and caprylic acids (for NLCs only) plus Pluronic F68 prepared for the encapsulation of DBC. We intended to provide a careful structural characterization of the nanoparticles to identify the relevant architectural parameters that lead to the desirable biological response. Initially, SLNs and NLCs were assessed in terms of their size distribution, morphology, surface charge, and drug loading. Spectroscopic techniques (infrared spectroscopy and electron paramagnetic resonance, EPR) plus small-angle X-ray scattering (SAXS) provided information on the interactions between nanoparticle components and their structural organization. The sizes of nanoparticles were in the 180 nm range with low polydispersity and negative zeta values (-25 to -46 mV). The partition coefficient of DBC between nanoparticles and water at pH 8.2 was very high (>10(4)). EPR (with doxyl-stearate spin labels) data revealed the existence of lamellar arrangements inside the lipid nanoparticles, which was also confirmed by SAXS experiments. Moreover, the addition of DBC increased the molecular packing of both SLN and NLC lipids, indicative of DBC insertion between the lipids, in the milieu assessed by spin labels. Such structural information brings insights into understanding the molecular organization of these versatile drug-delivery systems which have already demonstrated their potential for therapeutic applications in pain control. (AU)

Processo FAPESP: 14/14457-5 - Carreadores baseados em lipídios (SLN/NLC e lipossomas com gradiente iônico) como estratégia para aumentar a encapsulação e a potência de anestésicos locais
Beneficiário:Eneida de Paula
Linha de fomento: Auxílio à Pesquisa - Temático