Adaptation of Pseudomonas aeruginosa to the chronic phenotype by mutations in the algTmucABD operon in isolates from Brazilian cystic fibrosis patients

Autor(es)

Cacador, Natalia Candido ^[1] ; da Costa Capizzani, Carolina Paulino ^[1] ; Monteiro Marin Torres, Lfdia Alice Gomes ^[2] ; Galetti, Renata ^[1] ; Ciofu, Oana ^[3] ; da Costa Darini, Ana Lticia ^[1] ; Flefiby, Niels ^{[3, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Pediat, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil

^[3] Univ Copenhagen, Dept Immunol & Microbiol, Copenhagen - Denmark

^[4] Univ Hosp, Rigshosp, Dept Clin Microbiol, Copenhagen - Denmark

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 11 NOV 29 2018.
• Citações Web of Science: 3

Resumo

Chronic lung infection by Pseudomonas aeruginosa is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. This is associated with the conversion of the non-mucoid to the mucoid phenotype. However, there is little information about the occurrence of alginate-producing P. aeruginosa in CF patients outside Europe and North America. The aim of the present study was to investigate mutations in the algTmucABD operon in mucoid and non-mucoid isolates from Brazilian CF patients. Twenty-seven mucoid and 37 non-mucoid isolates from 40 CF patients chronically infected by P. aeruginosa attending a CF reference center in Brazil were evaluated by sequence analysis. Mutations in mucA were observed in 93% of the mucoid isolates and 54% of the non-mucoid isolates. Among these non-mucoid isolates, 55% were considered revertants, since they also had mutations in algT (algU). Most isolates associated with moderate alginate production presented point mutations in mucB and/or mucD. We identified 30 mutations not previously described in the operon. In conclusion, mutations in mucA were the main mechanism of conversion to mucoidy, and most of the non-mucoid isolates were revertants, but the mechanism of revertance is not fully explained by changes in algT. (AU)

• Processo FAPESP: 13/13455-6 - Epidemiologia das infecções bacterianas em pacientes com fibrose cística envolvendo Achromobacter e bactérias do complexo Burkholderia cepacia
• Beneficiário: Carolina Paulino da Costa Capizzani
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 15/11728-0 - Sequenciamento completo de plasmídeos carreando genes de resistência a antibióticos em bactérias de interesse e análise do genoma de linhagens de Pseudomonas aeruginosa produtoras de SPM-1 e de KPC-2 por sequenciamento em larga escala
• Beneficiário: Renata Galetti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/14494-8 - Epidemiologia molecular de bactérias gram-negativas e genética da resistência a antibióticos
• Beneficiário: Ana Lúcia da Costa Darini
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/13358-0 - Epidemiologia clássica e molecular de Pseudomonas aeruginosa isoladas de pacientes com Fibrose Cística
• Beneficiário: Natália Candido Caçador
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto