Hydrogen peroxide-mediated oxidative stress induces inflammasome activation in term human placental explants

Autor(es)

Nunes, Priscila Rezeck ^[1] ; Serrao Peracoli, Maria Terezinha ^[2] ; Romao-Veiga, Mariana ^[2] ; Matias, Mariana Leticia ^[1] ; Ribeiro, Vanessa Rocha ^[1] ; Da Costa Fernandes, Celio Junior B. ^[2] ; Peracoli, Jose Carlos ^[1] ; Rodrigues, Jose Ricardo ^[1] ; De Oliveira, Leandro ^[1]

Afiliação do(s) autor(es)

^[1] Botucatu Sao Paulo State Univ Unesp, Med Sch, Dept Gynaecol & Obstet, BR-18618970 Sao Paulo - Brazil

^[2] Botucatu Sao Paulo State Univ Unesp, Inst Biosci, Dept Microbiol & Immunol, BR-18618691 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH; v. 14, p. 29-36, OCT 2018.
• Citações Web of Science: 0

Resumo

Background: The placenta is a multifunctional organ that can suffer with imbalances between pro- and antioxidant molecules, contributing for inflammatory imbalance. The inflammation generated by oxidative stress may induce inflammasome activation, an essential complex for pro-inflammatory cytokine production. Objective: The aim of this study was to evaluate whether hydrogen peroxide (H2O2) mediated oxidative stress induces inflammasome activation on placental explants. Study design: Tissue cultures of placental explants obtained from normotensive pregnant women were performed in different concentrations of H2O2. Gene expressions of NLRP3, caspase-1, IL-1 beta, TNF-alpha and IL-10 were evaluated by qPCR. Superoxide dismutase (SOD), catalase, Heat shock protein 70 (Hsp70), Caspase-1, TNF-alpha, IL-1 beta, IL-10 and human Chorionic Gonadotropin (hCG) were determined by ELISA. Results: Concentrations of catalase, Hsp70, hCG and SOD were higher in cultures with 100 and 1000 mu M H2O2 compared to controls. Gene and protein expressions of TNF-alpha and IL-1 beta were elevated in cultures with 1000 mu M H2O2 compared to controls. This concentration led to inflammasome activation, by increasing gene expressions of NLRP3, caspase-1 and IL-1 beta. In contrast, gene and protein expressions of IL-10 were reduced at 100 and 1000 mu M H2O2. Protein expression of caspase-1 was higher in cultures of 100 mu M H2O2 compared to controls. Treatment with Glybenclamide at 200 mu M was used to prevent NLRP3 inflammasome activation. This concentration reduced protein expression of caspase-1 compared to culture with only H2O2 and control cultures. Conclusions: Our results confirm that H2O2 induces oxidative stress on placental explants and demonstrate that cell responses to this stress involve inflammasome activation. (AU)

• Processo FAPESP: 14/25611-5 - Efeito do estresse oxidativo sobre autofagia em tecido placentário
• Beneficiário: Priscila Rezeck Nunes
• Modalidade de apoio: Bolsas no Brasil - Mestrado