| Full text | |
| Author(s): |
Nunes, Priscila Rezeck
[1]
;
Serrao Peracoli, Maria Terezinha
[2]
;
Romao-Veiga, Mariana
[2]
;
Matias, Mariana Leticia
[1]
;
Ribeiro, Vanessa Rocha
[1]
;
Da Costa Fernandes, Celio Junior B.
[2]
;
Peracoli, Jose Carlos
[1]
;
Rodrigues, Jose Ricardo
[1]
;
De Oliveira, Leandro
[1]
Total Authors: 9
|
| Affiliation: | [1] Botucatu Sao Paulo State Univ Unesp, Med Sch, Dept Gynaecol & Obstet, BR-18618970 Sao Paulo - Brazil
[2] Botucatu Sao Paulo State Univ Unesp, Inst Biosci, Dept Microbiol & Immunol, BR-18618691 Sao Paulo - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | PREGNANCY HYPERTENSION-AN INTERNATIONAL JOURNAL OF WOMENS CARDIOVASCULAR HEALTH; v. 14, p. 29-36, OCT 2018. |
| Web of Science Citations: | 0 |
| Abstract | |
Background: The placenta is a multifunctional organ that can suffer with imbalances between pro- and antioxidant molecules, contributing for inflammatory imbalance. The inflammation generated by oxidative stress may induce inflammasome activation, an essential complex for pro-inflammatory cytokine production. Objective: The aim of this study was to evaluate whether hydrogen peroxide (H2O2) mediated oxidative stress induces inflammasome activation on placental explants. Study design: Tissue cultures of placental explants obtained from normotensive pregnant women were performed in different concentrations of H2O2. Gene expressions of NLRP3, caspase-1, IL-1 beta, TNF-alpha and IL-10 were evaluated by qPCR. Superoxide dismutase (SOD), catalase, Heat shock protein 70 (Hsp70), Caspase-1, TNF-alpha, IL-1 beta, IL-10 and human Chorionic Gonadotropin (hCG) were determined by ELISA. Results: Concentrations of catalase, Hsp70, hCG and SOD were higher in cultures with 100 and 1000 mu M H2O2 compared to controls. Gene and protein expressions of TNF-alpha and IL-1 beta were elevated in cultures with 1000 mu M H2O2 compared to controls. This concentration led to inflammasome activation, by increasing gene expressions of NLRP3, caspase-1 and IL-1 beta. In contrast, gene and protein expressions of IL-10 were reduced at 100 and 1000 mu M H2O2. Protein expression of caspase-1 was higher in cultures of 100 mu M H2O2 compared to controls. Treatment with Glybenclamide at 200 mu M was used to prevent NLRP3 inflammasome activation. This concentration reduced protein expression of caspase-1 compared to culture with only H2O2 and control cultures. Conclusions: Our results confirm that H2O2 induces oxidative stress on placental explants and demonstrate that cell responses to this stress involve inflammasome activation. (AU) | |
| FAPESP's process: | 14/25611-5 - EFFECT OF OXIDATIVE STRESS ON AUTOPHAGY IN PLACENTAL TISSUE |
| Grantee: | Priscila Rezeck Nunes |
| Support Opportunities: | Scholarships in Brazil - Master |