Relationship between Fusobacterium nucleatum, inflammatory mediators and microRNAs in colorectal carcinogenesis

Autor(es)

Proenca, Marcela Alcantara ^[1] ; Biselli, Joice Matos ^[1] ; Succi, Maysa ^[1] ; Severino, Fabio Eduardo ^[2] ; Berardinelli, Gustavo Noriz ^[3] ; Caetano, Alaor ^[4] ; Reis, Rui Manuel ^{[3, 5, 6]} ; Hughes, David J. ^[7] ; Silva, Ana Elizabete ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Dept Biol, Campus Sao Jose do Rio Preto, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Fac Med, Dept Surg & Orthoped, BR-18618687 Botucatu, SP - Brazil

^[3] Barretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Barretos, SP - Brazil

^[4] Endoscopy Ctr Rio Preto, BR-15015700 Sao Jose Do Rio Preto, SP - Brazil

^[5] Univ Minho, Life & Hlth Sci Res Inst, Campus Gualtar, P-4710057 Braga - Portugal

^[6] ICVS 3Bs PT Govt Associate Lab, Campus Gualtar, P-4710057 Braga - Portugal

^[7] Univ Coll Dublin, UCD Conway Inst, Canc Biol & Therapeut Grp, Dublin D04 V1W8 - Ireland

• Tipo de documento: Artigo Científico
• Fonte: WORLD JOURNAL OF GASTROENTEROLOGY; v. 24, n. 47, p. 5351-5365, DEC 21 2018.
• Citações Web of Science: 9

Resumo

AIM To examine the effect of Fusobacterium nucleatum (F.nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs (miRNAs). METHODS Levels of F. nucleatum DNA, cytokine gene mRNA (TLR2, TLR4, NFKB1, TNF, IL1B, IL6 and IL8), and potentially interacting miRNAs (miR-21-3p, miR-22-3p, miR-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction (qPCR) TaqMan (R) assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma (CRA) and 43 colorectal cancer (CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability (MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network. RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA (51.8%) and more markedly in CRC (72.1%). We observed significantly greater expression of TLR4, IL1B, IL8, and miR-135b in CRA lesions and TLR2, IL1B, IL6, IL8, miR-34a and miR-135b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1B, IL6, IL8 and miR-22 was positively correlated with F nucleatum quantification in CRC tumours. The mRNA expression of miR-135b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC. CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible miRNA-mediated activation of TLR2/TLR4. (AU)

• Processo FAPESP: 15/21464-0 - Avaliação do efeito da inflamação em mediadores da via de sinalização de TNF-a, na resposta ao dano do DNA e redes de interação com miRNAs na carcinogênese gástrica
• Beneficiário: Ana Elizabete Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular