Exercise Training Potentiates The Cardioprotective Effects of Stem Cells Post-infarction

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Vieira, Stella de Souza ^[1] ; Antonio, Ednei Luiz ^[1] ; de Melo, Brunno Lemes ^[1] ; Portes, Leslie Andrews ^{[1, 2]} ; Montemor, Jairo ^[1] ; Oliveira, Helenita Antonia ^[3] ; Martins, Flavia Leticia ^[4] ; Zogbi, Camila ^[4] ; Girardi, Adriana Costa ^[4] ; Silva, Jr., Jose Antonio ^[3] ; Camillo de Carvalho, Paulo de Tarso ^[3] ; Ferreira Tucci, Paulo Jose ^[1] ; Serra, Andrey Jorge ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Cardiol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Adventist Ctr, Sao Paulo - Brazil

^[3] Univ Nove Julho, Lab Biophoton, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Heart Inst InCor, Lab Genet & Mol Cardiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HEART LUNG AND CIRCULATION; v. 28, n. 2, p. 263-271, FEB 2019.
• Citações Web of Science: 3

Resumo

Background Preconditioning of cell recipients may exert a significant role in attenuating the hostility of the infarction milieu, thereby enhancing the efficacy of cell therapy. This study was conducted to examine whether exercise training potentiates the cardioprotective effects of adipose-derived stern cell (ADSC) transplantation following myocardial infarction (MI) in rats. Methods Four groups of female Fisher-344 rats were studied: Sham; non-trained rats with MI (sMI); non-trained rats with Ml submitted to ADSCs transplantation (sADSC); trained rats with Ml submitted to ADSCs (tADSC). Rats were trained 9 weeks prior to MI and ADSCs transplantation. Echocardiography was applied to assess cardiac function. Myocardial performance was evaluated in vitro. Protein expression analyses were carried out by immunoblotting. Periodic acid-Schiff staining was used to analyse capillary density and apoptosis was evaluated with terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. Results Echocardiography performed 4 weeks after the infarction revealed attenuated scar size in the both sADSC and tADSC groups compared to the sMI group. However, fractional shortening was improved only in the tADSC group. In vitro myocardial performance was similar between the tADSC and Sham groups. The expression of phosphoSer473Akt1 and VEGF were found to be higher in the hearts of the tADSC group compared to both the sADSC and sMI groups. Histologic analysis demonstrated that tADSC rats had higher capillary density in the remote and border zones of the infarcted sites compared to the sMI rats. Conclusions Preconditioning with exercise induces a pro-angiogenic milieu that may potentiate the therapeutic effects of ADSCs on cardiac remodelling following MI. (AU)

• Processo FAPESP: 15/11028-9 - A modalidade de treinamento físico aeróbio pode determinar a existência de remodelamento cardíaco: identificando peculiaridades estruturais, funcionais e moleculares dos modelos experimentais de natação e corrida
• Beneficiário: Andrey Jorge Serra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/10619-8 - Dipeptidil peptidase IV como um potencial alvo para a terapia da insuficiência cardíaca
• Beneficiário: Adriana Castello Costa Girardi
• Modalidade de apoio: Auxílio à Pesquisa - Regular