Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exercise Training Potentiates The Cardioprotective Effects of Stem Cells Post-infarction

Full text
Author(s):
Show less -
Vieira, Stella de Souza [1] ; Antonio, Ednei Luiz [1] ; de Melo, Brunno Lemes [1] ; Portes, Leslie Andrews [1, 2] ; Montemor, Jairo [1] ; Oliveira, Helenita Antonia [3] ; Martins, Flavia Leticia [4] ; Zogbi, Camila [4] ; Girardi, Adriana Costa [4] ; Silva, Jr., Jose Antonio [3] ; Camillo de Carvalho, Paulo de Tarso [3] ; Ferreira Tucci, Paulo Jose [1] ; Serra, Andrey Jorge [1, 3]
Total Authors: 13
Affiliation:
[1] Univ Fed Sao Paulo, Div Cardiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Adventist Ctr, Sao Paulo - Brazil
[3] Univ Nove Julho, Lab Biophoton, Sao Paulo - Brazil
[4] Univ Sao Paulo, Heart Inst InCor, Lab Genet & Mol Cardiol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: HEART LUNG AND CIRCULATION; v. 28, n. 2, p. 263-271, FEB 2019.
Web of Science Citations: 3
Abstract

Background Preconditioning of cell recipients may exert a significant role in attenuating the hostility of the infarction milieu, thereby enhancing the efficacy of cell therapy. This study was conducted to examine whether exercise training potentiates the cardioprotective effects of adipose-derived stern cell (ADSC) transplantation following myocardial infarction (MI) in rats. Methods Four groups of female Fisher-344 rats were studied: Sham; non-trained rats with MI (sMI); non-trained rats with Ml submitted to ADSCs transplantation (sADSC); trained rats with Ml submitted to ADSCs (tADSC). Rats were trained 9 weeks prior to MI and ADSCs transplantation. Echocardiography was applied to assess cardiac function. Myocardial performance was evaluated in vitro. Protein expression analyses were carried out by immunoblotting. Periodic acid-Schiff staining was used to analyse capillary density and apoptosis was evaluated with terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay. Results Echocardiography performed 4 weeks after the infarction revealed attenuated scar size in the both sADSC and tADSC groups compared to the sMI group. However, fractional shortening was improved only in the tADSC group. In vitro myocardial performance was similar between the tADSC and Sham groups. The expression of phosphoSer473Akt1 and VEGF were found to be higher in the hearts of the tADSC group compared to both the sADSC and sMI groups. Histologic analysis demonstrated that tADSC rats had higher capillary density in the remote and border zones of the infarcted sites compared to the sMI rats. Conclusions Preconditioning with exercise induces a pro-angiogenic milieu that may potentiate the therapeutic effects of ADSCs on cardiac remodelling following MI. (AU)

FAPESP's process: 15/11028-9 - The aerobic training method can determine the existence of cardiac remodeling: structural, functional and molecular analyzes of swimming and running experimental models.
Grantee:Andrey Jorge Serra
Support Opportunities: Regular Research Grants
FAPESP's process: 13/10619-8 - Dipeptidyl peptidase IV as a potential target for the therapy of heart failure
Grantee:Adriana Castello Costa Girardi
Support Opportunities: Regular Research Grants