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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Interleukin-10 negatively modulates extracellular signal-regulated kinases 1 and 2 in aorta from hypertensive mouse induced by angiotensin II infusion

Texto completo
Bressan, Alecsander F. [1] ; Fonseca, Gisele A. [2] ; Tostes, Rita C. [3] ; Webb, R. Clinton [4] ; Lima, Victor Vitorino [1] ; Giachini, Fernanda Regina [1, 2]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Fed Mato Grosso, Inst Biol & Hlth Sci, Av Valdon Varjao 6390, BR-78600000 Barra Do Garcas, Mato Grosso - Brazil
[2] Fed Univ Goias UFG, Inst Biol Sci ICS, Dept Pharmacol, Av Esperanca, Campus Samambaia, BR-74690900 Goiania, Go - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Av Bandeirantes, N3900, BR-14049900 Ribeirao Preto, SP - Brazil
[4] Augusta Univ, Dept Physiol, 1120 15th St, CA3126, Augusta, GA 30912 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: FUNDAMENTAL & CLINICAL PHARMACOLOGY; v. 33, n. 1, p. 31-40, FEB 2019.
Citações Web of Science: 0

The activation of extracellular signal-regulated kinase 1 and 2 (ERK 1/2) pathway promotes increased vascular contractility in angiotensin II (Ang II)-induced hypertensive mice. Interleukin-10 (IL-10) is an immune-regulatory cytokine with the ability to prevent vascular hypercontractility during hypertension. We hypothesized that IL-10 would downregulate vascular ERK 1/2 activation during Ang II-induced hypertension. Wild-type (WT) or IL-10 knockout (IL-10(-/-)) mice received Ang II infusion (90 eta g.min) or vehicle (saline), via osmotic mini-pumps (0.25 mu L/h for 14 days), whereas another WT group were infused with exogenous IL-10 (0.5 eta g/min, 14 days) simultaneously, or not, with Ang II. Aortic rings were mounted in a myograph, and concentration-response curves to phenylephrine were evaluated, in the presence or absence of ERK 1/2 inhibitor (PD98059, 10 mu m, 40 min). Protein expression of vascular ERK 1/2 was determined by Western blot. Ang II infusion increased the maximal contractile response in both WT and IL-10(-/-) mice. Concomitant infusion of IL-10 and Ang II prevented hypercontractility in the vasculature. Exogenous IL-10 infusion prevented ERK 1/2 activation and hypercontractility, induced by Ang II. These findings suggest that IL-10 negatively modulates ERK 1/2 activation and prevents hypercontractility during Ang II-induced hypertension. (AU)

Processo FAPESP: 10/52214-6 - Contribuição do estresse oxidativo e enzimas NADPH oxidases (NOXes) para as lesões vasculares e renais associadas ao diabetes
Beneficiário:Rita de Cassia Aleixo Tostes Passaglia
Linha de fomento: Auxílio à Pesquisa - Regular