| Full text | |
| Author(s): |
Bressan, Alecsander F.
[1]
;
Fonseca, Gisele A.
[2]
;
Tostes, Rita C.
[3]
;
Webb, R. Clinton
[4]
;
Lima, Victor Vitorino
[1]
;
Giachini, Fernanda Regina
[1, 2]
Total Authors: 6
|
| Affiliation: | [1] Univ Fed Mato Grosso, Inst Biol & Hlth Sci, Av Valdon Varjao 6390, BR-78600000 Barra Do Garcas, Mato Grosso - Brazil
[2] Fed Univ Goias UFG, Inst Biol Sci ICS, Dept Pharmacol, Av Esperanca, Campus Samambaia, BR-74690900 Goiania, Go - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, Av Bandeirantes, N3900, BR-14049900 Ribeirao Preto, SP - Brazil
[4] Augusta Univ, Dept Physiol, 1120 15th St, CA3126, Augusta, GA 30912 - USA
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | FUNDAMENTAL & CLINICAL PHARMACOLOGY; v. 33, n. 1, p. 31-40, FEB 2019. |
| Web of Science Citations: | 3 |
| Abstract | |
The activation of extracellular signal-regulated kinase 1 and 2 (ERK 1/2) pathway promotes increased vascular contractility in angiotensin II (Ang II)-induced hypertensive mice. Interleukin-10 (IL-10) is an immune-regulatory cytokine with the ability to prevent vascular hypercontractility during hypertension. We hypothesized that IL-10 would downregulate vascular ERK 1/2 activation during Ang II-induced hypertension. Wild-type (WT) or IL-10 knockout (IL-10(-/-)) mice received Ang II infusion (90 eta g.min) or vehicle (saline), via osmotic mini-pumps (0.25 mu L/h for 14 days), whereas another WT group were infused with exogenous IL-10 (0.5 eta g/min, 14 days) simultaneously, or not, with Ang II. Aortic rings were mounted in a myograph, and concentration-response curves to phenylephrine were evaluated, in the presence or absence of ERK 1/2 inhibitor (PD98059, 10 mu m, 40 min). Protein expression of vascular ERK 1/2 was determined by Western blot. Ang II infusion increased the maximal contractile response in both WT and IL-10(-/-) mice. Concomitant infusion of IL-10 and Ang II prevented hypercontractility in the vasculature. Exogenous IL-10 infusion prevented ERK 1/2 activation and hypercontractility, induced by Ang II. These findings suggest that IL-10 negatively modulates ERK 1/2 activation and prevents hypercontractility during Ang II-induced hypertension. (AU) | |
| FAPESP's process: | 10/52214-6 - Contribution of oxidative stress and NOXes to diabetes-associated vascular and renal injury |
| Grantee: | Rita de Cassia Aleixo Tostes Passaglia |
| Support Opportunities: | Regular Research Grants |