Aorta morphological analysis of ELA-2KO mice with abdominal aortic aneurysm induced by the infusion of Angiotensin II

• Grant number: 20/03308-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): April 01, 2020
• Effective date (End): March 31, 2023
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal researcher: Christiane Becari
• Grantee: Ariel Emiliano Souza do Couto
• Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 17/21539-6 - Role of renin angiotensin system in Abdominal Aortic Aneurysm, AP.JP

Abstract

The renin-angiotensin system (RAS) is involved in physiological and pathological processes. Nowadays, a new paradigm has emerged in which it is believed that two central axes modulate the function of the RAS: a deleterious axis composed of ACE/Ang II/AT1 receptor and another beneficial axis formed of ACE2/ Ang 1-7/MAS receptor. These functional aspects multiply the possible treatment targets for the treatment of cardiovascular diseases, including the abdominal aortic aneurysm (AAA). Experimental researches in mouse and rats indicate critical participation of the RAS in the pathogenesis of the AAA. An experimental model of formation of the AAA is through the chronic infusion of AngII in mice. This model of aneurysm simulates human disease in many aspects. One part of the project is related to the elastase-2 (ELA-2), an angiotensin II enzyme, in the vascular system. It will be interesting to elucidate the contribution of ELA-2 in the development of the AAA in the mice. Thus, This project aims induce AAA in wild mice (C57/B1-6) and elastase-2 knockout mice (ELA-2 KO) and to analyze modifications in aorta morphological. The experimental groups are I) Control C57/BI-6 mice; II) Control knockout ELA-2 mice; III) Induced aneurysm C57/BI-6 mice; IV) Induced aneurysm knockout ELA-2 mice.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

D'AVILA-MESQUITA, CAROLINA; COUTO, ARIEL E. S.; CAMPOS, LIGIA C. B.; VASCONCELOS, TAUANA F.; MICHELON-BARBOSA, JESSYCA; CORSI, CARLOS A. C.; MESTRINER, FABIOLA; PETROSKI-MORAES, BRUNO C.; GARBELLINI-DIAB, MARIA J.; COUTO, DANIEL M. S.; et al. MMP-2 and MMP-9 levels in plasma are altered and associated with mortality in COVID-19 patients. BIOMEDICINE & PHARMACOTHERAPY, v. 142, OCT 2021. (18/23718-8, 19/24369-0, 21/01195-6, 19/21721-4, 20/09999-4, 19/11485-1, 20/03308-0)