Age-based ultrasonographic criteria for diagnosis of autosomal dominant polycystic kidney disease in Persian cats

Autor(es)

Guerra, Juliana M. ^{[1, 2]} ; Freitas, Mariana F. ^[3] ; Daniel, Alexandre G. T. ^[4] ; Pellegrino, Arine ^{[3, 4]} ; Cardoso, Natalia C. ^[1] ; de Castro, Isac ; Onuchic, Luiz F. ^[5] ; Cogliati, Bruno ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

^[2] Adolfo Lutz Inst, Pathol Ctr, Nucleus Quantitat Pathol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Clin Med, Sao Paulo - Brazil

^[4] Gattos Specialized Feline Med Clin, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Sch Med, Disciplines Mol Med & Nephrol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FELINE MEDICINE AND SURGERY; v. 21, n. 2, p. 156-164, FEB 2019.
• Citações Web of Science: 1

Resumo

Objectives The aim of this study was to establish ultrasound criteria for the diagnosis of autosomal dominant polycystic kidney disease (ADPKD) in Persian cats. Methods Eighty-two Persian cats were assessed using renal ultrasound and genotyped for the C -> A transversion in exon 29 of PKD1. The animals were also submitted to hematological characterization, serum biochemistry analyses and urinalysis. Results Age, sex and neutering status did not differ between ADPKD (n = 12) and non-ADPKD (n = 70) cats. After integrated molecular genetics/ultrasonographic analysis, the presence of at least one renal cyst was sufficient to establish a diagnosis of ADPKD in animals up to 15 months of age. Two or more cysts were required for diagnosis in cats aged 16-32 months, and at least three cysts warranted diagnosis of ADPKD in animals aged 33-49 months. Finally, four or more cysts led to diagnosis in cats aged 50-66 months. Although cats with ADPKD exhibited higher serum calcium levels than non-affected cats, hematological, urinalysis and other biochemical parameters did not differ between the two groups. Conclusions and relevance Integrated analyses of imaging and molecular genetics data enabled, for the first time, the establishment of age-based ultrasonographic criteria for the diagnosis of ADPKD in Persian cats. The development of imaging criteria is particularly relevant and useful in the clinical setting given the current limitations to access and the cost of molecular genetics-based diagnostic tests. (AU)

• Processo FAPESP: 13/06471-5 - Estudo comparativo da extração de DNA em diferentes fontes de material biológico para a realização da reação em cadeia da polimerase (PCR) no diagnóstico da Doença Renal Policística em felinos da raça Persa
• Beneficiário: Natalia Cavalca Cardoso
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/19614-6 - Doença renal policística autossômica dominante em felinos da raça Persa: caracterização genotípica e fenotípica e elaboração de critérios para diagnóstico ultrassonográfico
• Beneficiário: Luiz Fernando Onuchic
• Modalidade de apoio: Auxílio à Pesquisa - Regular