MALDI-imaging analyses of honeybee brains exposed to a neonicotinoid insecticide

Autor(es)

Catae, Aline F. ^[1] ; da Silva Menegasso, Anally R. ^[1] ; Pratavieira, Marcel ^[1] ; Palma, Mario S. ^[1] ; Malaspina, Osmar ^[1] ; Roat, Thaisa C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, Ave 24-A, 1515 Bela Vista, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Pest Management Science; v. 75, n. 3, p. 607-615, MAR 2019.
• Citações Web of Science: 2

Resumo

BACKGROUND Toxicological studies evaluating the possible harmful effects of pesticides on bees are important and allow the emergence of protection and pollinator conservation strategies. This study aimed to evaluate the effects of exposure to a sublethal concentration of imidacloprid (LC50/100: 0.014651 ng imidacloprid mu L-1 diet) on the distribution of certain proteins identified in the brain of Apis mellifera worker bees using a MALDI-imaging approach. This technique enables proteomic analysis of tissues in situ by monitoring the spatiotemporal dynamics of the biochemical processes occurring at a specific time in specific brain neuropils. For this purpose, foraging bees were exposed to an 8-day diet containing a sublethal concentration of imidacloprid corresponding to the LC50/100. Bees were collected on day 8 of exposure, and their brains analyzed using protein density maps. RESULTS The results showed that exposure to imidacloprid led to a series of biochemical changes, including alterations in synapse regulation, apoptosis regulation and oxidative stress, which may adversely impair the physiology of these colony bees. CONCLUSION Worker bee contact with even tiny amounts of imidacloprid had potent effects leading to the overexpression of a series of proteins related to important cellular processes that were possibly damaged by the insecticide. (c) 2018 Society of Chemical Industry (AU)

• Processo FAPESP: 14/14070-3 - Efeitos do imidacloprido sobre a distribuição de proteínas no cérebro de abelhas Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini)
• Beneficiário: Aline Fernanda Catae
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/13370-8 - Como o cérebro de Apis mellifera (Lineu, 1758) (Hymenoptera, Apidae) responde à dose subletal de tiametoxam?
• Beneficiário: Thaisa Cristina Roat
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 12/50197-2 - Ação de produtos empregados no cultivo da cana-de-açúcar sobre organismos não alvos
• Beneficiário: Carmem Silvia Fontanetti Christofoletti
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático