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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MALDI-imaging analyses of honeybee brains exposed to a neonicotinoid insecticide

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Author(s):
Catae, Aline F. [1] ; da Silva Menegasso, Anally R. [1] ; Pratavieira, Marcel [1] ; Palma, Mario S. [1] ; Malaspina, Osmar [1] ; Roat, Thaisa C. [1]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci Rio Claro, Dept Biol, Ctr Study Social Insects, Ave 24-A, 1515 Bela Vista, BR-13506900 Rio Claro, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Pest Management Science; v. 75, n. 3, p. 607-615, MAR 2019.
Web of Science Citations: 2
Abstract

BACKGROUND Toxicological studies evaluating the possible harmful effects of pesticides on bees are important and allow the emergence of protection and pollinator conservation strategies. This study aimed to evaluate the effects of exposure to a sublethal concentration of imidacloprid (LC50/100: 0.014651 ng imidacloprid mu L-1 diet) on the distribution of certain proteins identified in the brain of Apis mellifera worker bees using a MALDI-imaging approach. This technique enables proteomic analysis of tissues in situ by monitoring the spatiotemporal dynamics of the biochemical processes occurring at a specific time in specific brain neuropils. For this purpose, foraging bees were exposed to an 8-day diet containing a sublethal concentration of imidacloprid corresponding to the LC50/100. Bees were collected on day 8 of exposure, and their brains analyzed using protein density maps. RESULTS The results showed that exposure to imidacloprid led to a series of biochemical changes, including alterations in synapse regulation, apoptosis regulation and oxidative stress, which may adversely impair the physiology of these colony bees. CONCLUSION Worker bee contact with even tiny amounts of imidacloprid had potent effects leading to the overexpression of a series of proteins related to important cellular processes that were possibly damaged by the insecticide. (c) 2018 Society of Chemical Industry (AU)

FAPESP's process: 14/14070-3 - Effects of imidacloprid on proteins distribution in the brain of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini)
Grantee:Aline Fernanda Catae
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/13370-8 - How is the reaction of the Apis mellifera brain to a sublethal dose of thiamethoxam?
Grantee:Thaisa Cristina Roat
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/50197-2 - Action of products used in cultivation of sugar cane on non-target organisms
Grantee:Carmem Silvia Fontanetti Christofoletti
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants