The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols

Autor(es)

Torres, Mariana A. ^[1] ; Monteiro, Matheus S. ^[1] ; Passarelli, Marina S. ^[1] ; Papa, Frederico O. ^[2] ; Dell'Aqua, Jr., Jose Antonio ^[2] ; Alvarenga, Marco Antonio ^[2] ; Martins, Simone M. M. K. ^[1] ; de Andrade, Andre F. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Swine Res Ctr, Sch Vet Med & Anim Sci, Pirassununga, SP - Brazil

^[2] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CRYOBIOLOGY; v. 86, p. 58-64, FEB 2019.
• Citações Web of Science: 0

Resumo

Boar semen cannot be immediately cryopreserved, it need be hold at 17 degrees C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 degrees C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17 degrees C. After each holding time (0, 4, 8, 12, 24, 28 and 32 h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32 h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24 h. (AU)

• Processo FAPESP: 15/17620-7 - O uso de holding time associado a protocolos de criopreservação do sêmen suíno
• Beneficiário: Matheus Saliba Monteiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/24690-4 - Assinaturas metabolômicas da influência do holding time sobre o aumento da criotolerância dos espermatozóides suínos
• Beneficiário: André Furugen Cesar de Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09441-8 - Assinaturas metabolômicas sobre à influência do holding time sobre o aumento da criotolerância dos espermatozoides suínos
• Beneficiário: Mariana Andrade Torres
• Modalidade de apoio: Bolsas no Brasil - Doutorado