The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols

Author(s)

Torres, Mariana A. ^[1] ; Monteiro, Matheus S. ^[1] ; Passarelli, Marina S. ^[1] ; Papa, Frederico O. ^[2] ; Dell'Aqua, Jr., Jose Antonio ^[2] ; Alvarenga, Marco Antonio ^[2] ; Martins, Simone M. M. K. ^[1] ; de Andrade, Andre F. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Swine Res Ctr, Sch Vet Med & Anim Sci, Pirassununga, SP - Brazil

^[2] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP - Brazil

• Document type: Journal article
• Source: CRYOBIOLOGY; v. 86, p. 58-64, FEB 2019.
• Web of Science Citations: 0

Abstract

Boar semen cannot be immediately cryopreserved, it need be hold at 17 degrees C prior to cryopreservation, holding time has been used to improve cryopreserved boar semen, since holding time allows a prolonged interaction between spermatozoa and seminal plasma components. However, until now only few periods of holding time have been studied, and boar semen had been held at 17 degrees C for 24 h to facilitate its manufacture. Thus, this experiment aims to study the effect several holding time (0, 4, 8, 12, 24, 28 and 32 h) on boar spermatozoa post thawed (PT) characteristics. Fifteen sperm-rich fractions of ejaculate were extended in Beltsville Thawing Solution and storage at 17 degrees C. After each holding time (0, 4, 8, 12, 24, 28 and 32 h), a sample was centrifuged, and sperm pellet was diluted in an extender composed of sugars, amino acids, buffers, 20% egg yolk (v/v), antibiotics, 2% glycerol as a cryoprotectant, and 2% methylformamide (v/v). Cryopreservation was performed with an automatic cryopreservation system. Cryopreserved boar semen was evaluated to spermatozoa kinetics, plasma and acrosomal membranes integrity, mitochondrial membrane potential, detection of superoxide anion, plasma membrane fluidity, and peroxidation. Twenty-four hours of holding increase total and progressive motility, rapid spermatozoa, and integrity of plasma and acrosome membranes. To mitochondrial membrane potential, 32 h is needed. However, holding time was not able to control the superoxide anion amount neither membrane lipid peroxidation, and had no effects on membrane fluidity. Thus, to reach the best results of PT boar semen the ideal holding time is 24 h. (AU)

• FAPESP's process: 15/17620-7 - The use of holding time associated with boar semen cryopreservation protocols
• Grantee: Matheus Saliba Monteiro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 16/24690-4 - Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance
• Grantee: André Furugen Cesar de Andrade
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/09441-8 - Metabolomics sgnatures on the influence of holding time on increasing boar spermatozoa cryotolerance
• Grantee: Mariana Andrade Torres
• Support Opportunities: Scholarships in Brazil - Doctorate