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Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance

Grant number: 16/24690-4
Support type:Regular Research Grants
Duration: August 01, 2017 - October 31, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:André Furugen Cesar de Andrade
Grantee:André Furugen Cesar de Andrade
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Heidge Fukumasu ; Simone Maria Massami Kitamura Martins

Abstract

Despite the great scientific investments, boar semen cryopreservation stills an important challenge for science, regarding post-thawed boar semen quality, regardless pre-cryopreservation quality or cryopreservation protocol used. Some proteins have been described as freezeability biological marks of boar semen. However, seminal plasma have a wide range components, so it lead us to believe that not only proteins but also lipids, ions and others components could be beneficial or detrimental to boar semen freezeability. Jointly, holding time (incubation time in own seminal plasma before cryopreservation) has been used to improve post-thawed boar semen quality. Holding time allows greater contact of spermatozoa with seminal plasma components, and it is reported to improve cryotolerance at low temperatures. It led us to postulate whether cryopreservation with or without holding time change the interaction between seminal plasma molecules and spermatozoa, and then freezeability. Thus, three experiments will be conducted in order to understand the influence of holding time on increasing boar spermatozoa cryotolerance. First experiment aims to determine the optimal holding time to boar semen cryopreservation. Second experiment aims to study the differences between boar spermatozoa freezeability and holding time. Finally, the third experiment aims to elucidated, through metabolomics, which seminal molecule contributes for the increasing in boar spermatozoa freezeability (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PASSARELLI, MARINA DA SILVA; PINOTI PAVANELI, ANA PAULA; RAVAGNANI, GISELE MOURO; MARTINS, MATHEUS PASINI; PEDROSA, ANA CAROLINA; MASSAMI KITAMURA MARTINS, SIMONE MARIA; DE ALCANTRA ROCHA, NATHALIA RAISSA; BITTAR RIGO, VICTOR HENRIQUE; YASUI, GEORGE; YESTE, MARC; CESAR DE ANDRADE, ANDRE FURUGEN. Effects of different equilibration times at 5 degrees C on boar sperm cryotolerance. Animal Reproduction Science, v. 219, AUG 2020. Web of Science Citations: 0.
TORRES, MARIANA A.; MONTEIRO, MATHEUS S.; PASSARELLI, MARINA S.; PAPA, FREDERICO O.; DELL'AQUA, JR., JOSE ANTONIO; ALVARENGA, MARCO ANTONIO; MARTINS, SIMONE M. M. K.; DE ANDRADE, ANDRE F. C. The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols. CRYOBIOLOGY, v. 86, p. 58-64, FEB 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.