Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Behavioral and stereological analysis of the prefrontal cortex of rats submitted to chronic alcohol intake

Texto completo
Autor(es):
Conte, Rafael [1] ; Lobo Ladd, Fernando Vagner [2] ; Barbosa Lobo Ladd, Aliny Antunes [3] ; Moreira, Amanda Lopez [3] ; Le Sueur-Maluf, Luciana [4] ; Viana, Milena de Barros [4] ; Cespedes, Isabel Cristina [5]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo UNIFESP, Dept Neurociencias & Neurocirurgia, Sao Paulo, SP - Brazil
[2] Univ Fed Rio Grande do Norte, Dept Morfol, Natal, RN - Brazil
[3] Univ Sao Paulo, Dept Cirurgia & Med Vet, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Biociencias, Santos, SP - Brazil
[5] Univ Fed Sao Paulo UNIFESP, Dept Morfol & Genet, Sao Paulo, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Behavioural Brain Research; v. 362, p. 21-27, APR 19 2019.
Citações Web of Science: 4
Resumo

Alcohol consumption has been identified as a causal factor promoting changes in different molecular and cellular mechanisms resulting in neurodegeneration. This process is specific to certain brain regions and its effects on different areas of the brain can result in a variety of deleterious consequences. The prefrontal cortex (PFC) appears to be particularly sensitive to alcohol-induced neurodegeneration; this region is quite complex, as it is responsible for high order mental processes such as decision making. Thus, it is important to have precise and unbiased data of neuronal morphology parameters to understand the real effects of alcohol on the PFC. This study aimed to investigate alcohol-induced neurodegeneration in the PFC by utilizing behavioral and stereological methods. In the first phase of the study, we utilized eighteen animals, six controls and twelve alcohol-treated, that were submitted to voluntary chronic alcohol ingestion for four or eight weeks. Their brains were analyzed by design-based stereology methods to assess number and volume parameters regarding neuronal integrity in regions of the PFC (prelimbic - PL, infralimbic - IL and anterior cingulate - ACC). In the second phase of the study, six animals were utilized as controls and eight animals were submitted to the same alcohol ingestion protocol and to a behavioral decision-making test. In conclusion, our findings indicate that chronic alcohol consumption promotes a decrease in volume in the prelimbic and in the anterior cingulate, a decrease of mean neuronal volume in the anterior cingulate cortex and a decrease of total volume of neurons in the IL area. We did not observe changes in decision-making behavior in either of the two periods of alcohol intake. This shows that morphological changes occur in specific regions of the prefrontal cortex, a noble area of cognitive functions, induced by chronic alcohol consumption. (AU)

Processo FAPESP: 13/01158-7 - Análise da influência do polimorfismo genético associado aos peptídeos e receptores da família CRF e do estresse ambiental no desenvolvimento da dependência de álcool
Beneficiário:Isabel Cristina Céspedes
Linha de fomento: Auxílio à Pesquisa - Regular