High fat diet reduces the expression of miRNA-29b in heart and increases susceptibility of myocardium to ischemia/reperfusion injury

Autor(es)

Guedes, Elaine Castilho ^[1] ; da Silva, Ivson Bezerra ^{[1, 2]} ; Lima, Vanessa Morais ^[1] ; Miranda, Juliane B. ^[1] ; Albuquerque, Ruda P. ^[3] ; Ferreira, Julio C. B. ^[3] ; Barreto-Chaves, Maria Luiza M. ^[1] ; Diniz, Gabriela Placona ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Cell Biol & Funct Anat, Av Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Fed Paraiba, Dept Morphol, Hlth Sci Ctr, Joao Pessoa, Paraiba - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Integrat Syst Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. 234, n. 6, p. 9399-9407, JUN 2019.
• Citações Web of Science: 2

Resumo

Several studies have shown the role of microRNAs (miRNAs) in myocardial dysfunction in response to ischemia/reperfusion (I/R). In this study, we investigated the impact of high fat (HF) diet in the myocardial susceptibility to I/R injury, as well as in the expression of miRNA-29b. Isolated heart experiments using the ex vivo Langendorff perfusion model were used to induce cardiac I/R injury. HF diet-induced cardiac hypertrophy and impaired cardiac functional recovery after I/R. miRNA-29b, which targets Col1, was reduced in the heart of HF diet-fed mice, whereas the cardiac expression of Col1 was increased. In addition, hypoxia-reoxygenation (H/R) reduced the expression of miRNA-29b in cardiomyoblasts cultures. However, the overexpression of miRNA-29b in cardiomyoblasts reduced p53 mRNA levels and H/R injury, suggesting that downregulation of miRNA-29b may be involved in I/R injury. Together, our findings suggest that the reduced expression of miRNA-29b may be involved in the deteriorated cardiac functional recovery following I/R in obese mice. (AU)

• Processo FAPESP: 15/21859-5 - Papel do microRNA-22 na instalação das alterações cardíacas e metabólicas induzidas pela dieta rica em gordura
• Beneficiário: Gabriela Placoná Diniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/16694-2 - Regulação intermolecular da proteína DICER pelo aldeído 4-hidroxinonenal: uma abordagem translacional
• Beneficiário: Julio Cesar Batista Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/05742-5 - Efeito da dieta rica em gordura no padrão de expressão de microRNAs no tecido cardíaco
• Beneficiário: Gabriela Placoná Diniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular