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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

High fat diet reduces the expression of miRNA-29b in heart and increases susceptibility of myocardium to ischemia/reperfusion injury

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Author(s):
Guedes, Elaine Castilho [1] ; da Silva, Ivson Bezerra [1, 2] ; Lima, Vanessa Morais [1] ; Miranda, Juliane B. [1] ; Albuquerque, Ruda P. [3] ; Ferreira, Julio C. B. [3] ; Barreto-Chaves, Maria Luiza M. [1] ; Diniz, Gabriela Placona [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Cell Biol & Funct Anat, Av Prof Lineu Prestes 2415, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Fed Paraiba, Dept Morphol, Hlth Sci Ctr, Joao Pessoa, Paraiba - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Lab Integrat Syst Biol, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Journal of Cellular Physiology; v. 234, n. 6, p. 9399-9407, JUN 2019.
Web of Science Citations: 2
Abstract

Several studies have shown the role of microRNAs (miRNAs) in myocardial dysfunction in response to ischemia/reperfusion (I/R). In this study, we investigated the impact of high fat (HF) diet in the myocardial susceptibility to I/R injury, as well as in the expression of miRNA-29b. Isolated heart experiments using the ex vivo Langendorff perfusion model were used to induce cardiac I/R injury. HF diet-induced cardiac hypertrophy and impaired cardiac functional recovery after I/R. miRNA-29b, which targets Col1, was reduced in the heart of HF diet-fed mice, whereas the cardiac expression of Col1 was increased. In addition, hypoxia-reoxygenation (H/R) reduced the expression of miRNA-29b in cardiomyoblasts cultures. However, the overexpression of miRNA-29b in cardiomyoblasts reduced p53 mRNA levels and H/R injury, suggesting that downregulation of miRNA-29b may be involved in I/R injury. Together, our findings suggest that the reduced expression of miRNA-29b may be involved in the deteriorated cardiac functional recovery following I/R in obese mice. (AU)

FAPESP's process: 17/16694-2 - Impact of 4-hydroxinonenal on DICER regulation: a translational approach
Grantee:Julio Cesar Batista Ferreira
Support type: Regular Research Grants
FAPESP's process: 15/21859-5 - Role of microRNA-22 in the development of cardiac and metabolic alterations induced by high fat diet
Grantee:Gabriela Placoná Diniz
Support type: Regular Research Grants
FAPESP's process: 13/05742-5 - Effect of the high fat diet on the microRNA expression pattern in the cardiac tissue
Grantee:Gabriela Placoná Diniz
Support type: Regular Research Grants