Cytotoxic effects of Euterpe oleraceae fruit oil (açaí) in rat liver and thyroid tissues

Autor(es)

Marques, Eduardo de Souza ^[1] ; Froder, Juliano Gabriel ^[1] ; de Oliveira, Patricia Rosa ^[2] ; Perazzo, Fabio Ferreira ^[3] ; Pires Rosa, Paulo Cesar ^[4] ; de Mascarenhas Gaivao, Isabel O'Neill ^{[5, 6]} ; Camargo Mathias, Maria Izabel ^[2] ; Maistro, Edson Luis ^{[7, 1]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Programa Posgrad Biol Geral & Aplicada, Inst Biociencias, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista, Dept Biol, Inst Biociencias, Rio Claro, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Diadema, SP - Brazil

^[4] Univ Estadual Campinas, Fac Ciencias Med, Campinas, SP - Brazil

^[5] Univ Tras os Montes & Alto Douro, Escola Ciencias Agr & Vet, Vila Real - Portugal

^[6] Univ Tras os Montes & Alto Douro, Dept Genet & Biotecnol, Vila Real - Portugal

^[7] Univ Estadual Paulista, Dept Fonoaudiol, Fac Filosofia & Ciencias, Marilia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 29, n. 1, p. 54-61, JAN-FEB 2019.
• Citações Web of Science: 0

Resumo

Abstract Euterpe oleracea Mart., Arecaceae, fruit (açaí) presents considerable potential for the development of new medicines due to its phytochemical composition and antioxidant activity. More recently, special attention has been given to the pharmacological potential of the fruit's oil. This study analysed the histological and histochemical effects of different dosages of açaí oil on rat's liver and thyroid cells, in order to evaluate its cytotoxic potential after administration for consecutive days. Male Wistar rats were treated with the açaí oil by gavage at doses of 30, 100 and 300 mg/kg, for 14 days, within a 24 h interval. Liver and thyroid fragments were collected for histology (hematoxylin and eosin) and histochemistry analysis (blue of Nilo (lipids), Baker (lipids), bromophenol blue (protein), PAS (polysaccharides)). The results showed that animals exposed to açaí oil presented alterations in the liver cells, where the integrity of the liver tissue was increasingly lost as the açaí oil doses increased. Nuclear pyknosis was observed in several hepatocytes, evidencing the occurrence of cell death. Alteration in the amount of lipids, polysaccharides, vacuoles in the cytoplasm, and proliferation of Kupffer cells were observed in histochemical analyzes. As for the thyroid of the treated rats, alterations were observed in the size of the follicular lumen and also in the connective tissue found between the follicles. Under the experimental conditions employed in the present study, the cytotoxicity observed in this work is worrying, specially considering the liver, when frequent or continuous damage could lead pathological disorders in this organ. (AU)

• Processo FAPESP: 12/17241-8 - Investigação dos efeitos citotóxico, genotóxico, antigenotóxico e antioxidante do óleo de Euterpe oleracea Mart. (açaí): análises in vitro e in vivo, histológicas, ultra-estruturais e hematológicas
• Beneficiário: Edson Luis Maistro
• Modalidade de apoio: Auxílio à Pesquisa - Regular